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Extraction process for lychee seed saponin

An extraction process, nucleoside technology, applied in plant raw materials, metabolic diseases, plant/algae/fungus/moss components, etc., can solve the problem of low extraction efficiency of saponins, time-consuming water extraction and alcohol precipitation, and many impurities in the extract etc. to achieve the effect of saving time and solvent, less solvent consumption, and reduced extraction time

Active Publication Date: 2011-05-11
GUANGZHOU HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the separation and preparation of litchi core components mainly use water extraction and alcohol precipitation and solvent extraction.
These extraction processes are not efficient for saponin extraction
The present invention aims to provide a process for extracting saponins from litchi cores with high extraction rate and simple process flow aiming at the deficiencies of the existing litchi core chemical components extraction process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The lychee core is crushed, and the particle size is 20-30 mesh. Take 50g of litchi kernel powder, add 150ml of water (equivalent to 3 times the weight of litchi kernel powder) to soak for 10min, weigh 0.5g of cellulase, 0.5g of pectinase and 0.5g of dextranase, add it, mix well, The temperature was controlled at 50°C, and the enzymatic hydrolysis treatment was carried out for 3 hours. Then add 150ml of 70% ethanol solution, and extract for 40min under the condition of ultrasonic frequency of 20KHz. The extract was filtered and concentrated under reduced pressure to obtain 4.53 g of litchi saponin crude extract. Pass the crude extract of litchi nucleosides through a macroporous adsorption resin column, and elute with 70% ethanol solution for 3 column volumes to obtain 3.85 g of litchi nucleosides. After determination, the saponin content of litchi nuclei was 85.0%.

Embodiment 2

[0022] The lychee core is crushed, and the particle size is 20-30 mesh. Take 50g of lychee seed powder, add 150ml of water to soak for 5min, weigh 0.5g of cellulase and 0.5g of pectinase, add it, mix well, control the temperature at 50°C, and enzymatically treat for 3 hours. Then add 150ml of 70% ethanol solution, and extract for 40min under the condition of ultrasonic frequency of 20KHz. The extract was filtered and concentrated under reduced pressure to obtain 4.20 g of litchi saponin crude extract. The crude extract of litchi nucleosides was passed through a macroporous adsorption resin column, and eluted with 70% ethanol solution for 3 column volumes to obtain 3.55 g of litchi nucleosides. After determination, the saponin content of litchi nuclei was 83.5%.

Embodiment 3

[0024] The lychee core is crushed, and the particle size is 10-20 mesh. Take 50g of lychee seed powder, add 150ml of water to soak for 10min, weigh 0.5g of cellulase and 0.5g of dextranase into it, mix well, control the temperature at 50°C, and perform enzymatic hydrolysis for 3 hours. Then add 150ml of 70% ethanol solution, and extract for 40min under the condition of ultrasonic frequency of 20KHz. The extract was filtered and concentrated under reduced pressure to obtain 4.03 g of litchi saponin crude extract. Pass the crude extract of litchi nucleosides through a macroporous adsorption resin column, and elute with 70% ethanol solution for 3 column volumes to obtain 3.48 g of litchi nucleosides. After determination, the saponin content of litchi nuclei was 83.9%.

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PUM

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Abstract

The invention discloses an extraction process for lychee seed saponin. The process comprises the following steps: 1) lychee seeds are pulverized, a certain amount of lychee seed powder is weighted and is soaked with water, and combinative enzyme reagent, which accounts for 1 to 2 percent of the weight of the lychee seed powder, is added in for enzymolysis treatment; 2) ethanol solution is added in for ultrasonic extraction, so as to obtain ultrasonic extracting solution; 3) filtering treatment and vacuum concentration treatment are conducted to the ultrasonic extracting solution, so as to obtain lychee seed saponin coarse extractive; and 4) macroporous adsorption resin is adopted to sublimate the lychee seed saponin coarse extractive, so as to obtain the lychee seed saponin. In the process, cell walls are softened and degraded through enzymes; with the combination of ultrasonic cavitation, the cell walls are fully destroyed, and the dissolution of active ingredients is accelerated and increased; and through the nonpolar macroporous adsorption resin, saponin ingredients of the lychee seeds are concentrated and separated, so as to prepare the high-content lychee seed saponin. The process has the advantages of time and labor conservation, lower cost and the like.

Description

technical field [0001] The invention relates to a litchi saponin extraction process, which belongs to the technical field of traditional Chinese medicine extraction. Background technique [0002] Lychee Seed (Litchi Seed, Litchi Seed), also known as Li Ren, Li He, Da Li He, is the dry mature seed of Sapindaceae plant Litchi (Litchi Chinensis Sonn), warm in nature, slightly sweet, bitter, astringent, no poison. "Compendium of Materia Medica" records that lychee core "quenches thirst, benefits people's color, and eats it to relieve polydipsia" (polydipsia, modern medicine is called diabetes). Studies have shown that lychee core has the functions of lowering blood sugar, regulating blood lipids, improving anti-oxidation, and inhibiting the surface antigen of hepatitis B virus. Experimental research and clinical treatment have shown that lychee core extract can significantly lower blood sugar and effectively treat diabetes. It is of great social significance to make full use ...

Claims

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Application Information

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IPC IPC(8): A61K36/77C12P19/44A61P3/10A61K131/00
CPCA61K36/77A61K2236/19A61P3/10
Inventor 郭洁文叶碧波
Owner GUANGZHOU HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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