Extraction process for lychee seed saponin

An extraction process, nucleoside technology, applied in plant raw materials, metabolic diseases, plant/algae/fungus/moss components, etc., can solve the problem of low extraction efficiency of saponins, time-consuming water extraction and alcohol precipitation, and many impurities in the extract etc. to achieve the effect of saving time and solvent, less solvent consumption, and reduced extraction time

Active Publication Date: 2011-05-11
GUANGZHOU HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the separation and preparation of litchi core components mainly use water extraction and alcohol precipitation and solvent extraction.
These extraction processes are not efficient for saponin extraction
The pr

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example one:

[0020] The lychee core is crushed to a particle size of 20-30 mesh. Take 50g lychee core powder, add 150ml of water (equivalent to 3 times the weight of lychee core powder) and soak for 10min, weigh 0.5g cellulase, 0.5g pectinase and 0.5g glucanase into it, mix well, Control the temperature at 50°C and enzymatically treat for 3 hours. Then add 150ml of 70% ethanol solution, and extract for 40min under the condition of ultrasonic frequency of 20KHz. The extract was filtered and concentrated under reduced pressure to obtain 4.53 g of lychee saponins crude extract. The crude lychee saponins were passed through a macroporous adsorption resin column and eluted with a 70% ethanol solution for 3 column volumes to obtain 3.85 g of lychee saponins. After determination, the content of lychee saponins is 85.0%.

Example Embodiment

[0021] Embodiment two:

[0022] The lychee core is crushed to a particle size of 20-30 mesh. Take 50g of lychee kernel powder, add 150ml of water to soak for 5min, weigh 0.5g of cellulase and 0.5g of pectinase into it, mix well, control the temperature at 50℃, and treat the enzyme for 3 hours. Then add 150ml of 70% ethanol solution, and extract for 40min under the condition of ultrasonic frequency of 20KHz. The extract was filtered and concentrated under reduced pressure to obtain 4.20 g of lychee saponins crude extract. The crude lychee saponins were passed through a macroporous adsorption resin column and eluted with a 70% ethanol solution for 3 column volumes to obtain 3.55 g of lychee saponins. It was determined that the content of lychee saponins was 83.5%.

Example Embodiment

[0023] Embodiment three:

[0024] The lychee core is crushed to a particle size of 10-20 mesh. Take 50g of lychee kernel powder, add 150ml of water to soak for 10min, weigh 0.5g of cellulase and 0.5g of glucanase into it, mix well, control the temperature at 50℃, and treat the enzyme for 3 hours. Then add 150ml of 70% ethanol solution, and extract for 40min under the condition of ultrasonic frequency of 20KHz. The extract was filtered and concentrated under reduced pressure to obtain 4.03 g of crude lychee saponins. The crude lychee saponins were passed through a macroporous adsorption resin column and eluted with a 70% ethanol solution for 3 column volumes to obtain 3.48 g of lychee saponins. It was determined that the content of lychee saponins was 83.9%.

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PUM

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Abstract

The invention discloses an extraction process for lychee seed saponin. The process comprises the following steps: 1) lychee seeds are pulverized, a certain amount of lychee seed powder is weighted and is soaked with water, and combinative enzyme reagent, which accounts for 1 to 2 percent of the weight of the lychee seed powder, is added in for enzymolysis treatment; 2) ethanol solution is added in for ultrasonic extraction, so as to obtain ultrasonic extracting solution; 3) filtering treatment and vacuum concentration treatment are conducted to the ultrasonic extracting solution, so as to obtain lychee seed saponin coarse extractive; and 4) macroporous adsorption resin is adopted to sublimate the lychee seed saponin coarse extractive, so as to obtain the lychee seed saponin. In the process, cell walls are softened and degraded through enzymes; with the combination of ultrasonic cavitation, the cell walls are fully destroyed, and the dissolution of active ingredients is accelerated and increased; and through the nonpolar macroporous adsorption resin, saponin ingredients of the lychee seeds are concentrated and separated, so as to prepare the high-content lychee seed saponin. The process has the advantages of time and labor conservation, lower cost and the like.

Description

technical field [0001] The invention relates to a litchi saponin extraction process, which belongs to the technical field of traditional Chinese medicine extraction. Background technique [0002] Lychee Seed (Litchi Seed, Litchi Seed), also known as Li Ren, Li He, Da Li He, is the dry mature seed of Sapindaceae plant Litchi (Litchi Chinensis Sonn), warm in nature, slightly sweet, bitter, astringent, no poison. "Compendium of Materia Medica" records that lychee core "quenches thirst, benefits people's color, and eats it to relieve polydipsia" (polydipsia, modern medicine is called diabetes). Studies have shown that lychee core has the functions of lowering blood sugar, regulating blood lipids, improving anti-oxidation, and inhibiting the surface antigen of hepatitis B virus. Experimental research and clinical treatment have shown that lychee core extract can significantly lower blood sugar and effectively treat diabetes. It is of great social significance to make full use ...

Claims

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Application Information

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IPC IPC(8): A61K36/77C12P19/44A61P3/10A61K131/00
CPCA61K36/77A61K2236/19A61P3/10
Inventor 郭洁文叶碧波
Owner GUANGZHOU HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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