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Method for extracting tabersonine from voacanga seed

A technology of horse boll fruit and its boning, which is applied in the field of alkaloid extraction and separation, can solve the problems of complicated process, low yield and high cost, and achieve the effects of reducing pollution, good quality and improving yield

Active Publication Date: 2011-05-11
SHAANXI JIAHE PHYTOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a process for preparing Taborin to overcome the problems of cumbersome process in the prior art, high cost, low yield, and the use of solvents that are seriously harmful to the human body.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 50kg of potato seeds and grind them to 30 meshes, and extract them with 100kg of 0.5% sulfuric acid aqueous solution at 60°C with stirring for 3 times to obtain an extract.

[0030] The above three extracts were combined, the pH of the solution was adjusted to 10 with ammonia water, the precipitate was placed overnight, centrifuged, and the supernatant was discarded to obtain a precipitate with a wet weight of 5 kg.

[0031] Add 20kg of water to the above wet precipitate and stir to form a suspension, then extract with 25kg of petroleum ether for a total of 3 times to obtain the extract. The three extracts were combined and concentrated to 2.5kg of oily substance, and the oily substance was put in the refrigerator overnight, and crystals were precipitated in the oil, and then centrifuged and dried to obtain 1.35kg of light yellow powder, which was Taborin, with a yield of 2.7% and a content of 96.6%. .

Embodiment 2

[0033] Take 50 kg of potato seeds and grind them to 30 meshes, and extract them with 150 kg of 3% hydrochloric acid aqueous solution at 80°C with stirring for 3 times to obtain an extract.

[0034] The above three extracts were combined, the pH of the solution was adjusted to 9 with 25% sodium carbonate aqueous solution, the precipitate was placed overnight, centrifuged, the supernatant was discarded, and the precipitate was obtained. The wet weight of the precipitate was 6.3 kg.

[0035] Add 25.2kg of water to the above wet precipitate and stir to form a suspension, then extract with 31.5kg of petroleum ether: n-hexane = 1:1 (volume ratio), and extract 3 times in total to obtain the extract. The three extracts were combined and concentrated to 3kg of oily substance, and the oily substance was put in the refrigerator overnight, crystals were precipitated in the oil, and centrifuged and dried to obtain 1.5kg of light yellow powder, which was Taborin, with a yield of 3% and a con...

Embodiment 3

[0037] Take 50kg of potato seeds and grind them to 30 meshes, and extract them with 250kg of 5% citric acid aqueous solution at 80°C with stirring for 3 times to obtain an extract.

[0038] The above three extracts were combined, and the pH of the solution was adjusted to 11 with a 20% aqueous sodium hydroxide solution. The precipitate was placed overnight, centrifuged, and the supernatant was discarded to obtain a precipitate. The wet weight of the precipitate was 5.7 kg.

[0039] Add 22.8kg of water to the above wet precipitate and stir to form a suspension, then extract with 28.5kg of petroleum ether: n-hexane = 3:1 (volume ratio), and extract 3 times in total to obtain the extract. The three extracts were combined and concentrated to 2.5kg of oily matter, and the oily matter was put in the refrigerator overnight, crystals were precipitated in the oil, and centrifuged and dried to obtain 1.2kg of light yellow powder, which was Taborin, with a yield of 2.4% and a content of 9...

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Abstract

The invention discloses a process for preparing tabersonine, aiming at solving the problems of tedious process, higher cost, lower yield and the use of solvents serious harmful to human bodies in the prior art. The process comprises the steps of: crushing raw materials, extracting with acid water, alkalizing and extracting the extracting solution, mixing an organic extraction layer, concentrating, recycling until no solvent exists, placing in a refrigerator, standing overnight, precipitating crystals, centrifuging, and drying the crystals. In method disclosed by the invention, the properties of alkaloid are utilized for acidizing tabersonine into salt with a water extraction process, thus, the production steps are simplified, and the production cost is reduced; the method has the advantages of simple process and easiness for operation, the yield is largely improved by about 3%, and the products are light yellow powder and have higher quality; and in addition, because less solvent is used, the pollution to the environment and the harm to human bodies are lowered.

Description

technical field [0001] The invention belongs to the field of alkaloid extraction and separation, and relates to a method for extracting taborin, in particular to a method for extracting taborin from potato seeds. Background technique [0002] Potato fruit (Voacanga) is an African plant, and an alkaloid can be isolated from its seeds, called tabersonine, also known as willow leaf water glycyrrhizin, and its chemical name is Aspidospermidine-3-carboxylic acid, 2,3,6,7-tetradehydro-(5α, 12β, 19α)-methylseter. Studies have shown that this component has a hypotensive effect, and its strength is 25% of that of reserpine, and it is also an important raw material for the synthesis of anticancer drug vinpocetine. [0003] Patent "A Preparation Process of Willow Leaf Glycyrrhizin" (Publication No. 101250188A), the seeds are crushed and then dried, extracted with organic solvent reflux, combined with the extracts to recover the organic solvent, acidified by adding hydrochloric acid, m...

Claims

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Application Information

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IPC IPC(8): C07D471/20
Inventor 王春德王晓莹肖红郭文华肖金霞
Owner SHAANXI JIAHE PHYTOCHEM
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