A Moderately Halophilic Inorganic Phosphorus Solubilizing Bacteria
A functional bacteria and inorganic technology, applied in the field of microorganisms, can solve the problem of lack of effective phosphorus bacteria, etc., to achieve the effect of reducing pH value and increasing the content of available phosphorus
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Embodiment 1
[0009] Screening, Purification and Preservation of Example 1 Bacterial Strains
[0010] Sample collection: The sampling site is the sediments off the coast of the Yellow Sea, China (36.50N, 123.82E). After the sample is collected, put it into a sterilized sealed polyethylene bag, put it in an ice box and bring it back to the laboratory, and store it at -20°C.
[0011] Strain screening and purification: Take 1g of the sample collected above and transfer it into 100ml sterilized seawater, shake evenly, and dilute it into different gradients of 10 -3 、10 -4 and 10 -5 , 100 μl was taken out and inoculated in high-salt inorganic phosphorus bacterial solid medium by coating method, each gradient was repeated three times, 28 ° C, 72 hours of culture, and the positive colonies that produced transparent circles were selected for further screening and purification verification. During the purification verification process, the culture condition was 28°C for 7 days, and the strain that...
Embodiment 2
[0015] Example 2 Inorganic Phosphorus Solubilizing Functional Bacteria YH8 Salinity and pH Value Growth Tolerance Test
[0016] 1. Salinity growth tolerance test
[0017] Salt gradient medium configuration: use salt-free LB medium as the base medium, add NaCl to it to obtain salinity of 0, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 8.0, 10.0, 12.0, 15.0 , 20.0, 25.0 and 30.0% (w / v) series of salinity gradient medium, pH value 7.8, sterilized at 115° C. for 30 minutes and cooled for later use.
[0018] The formula of the salt-free LB medium is: peptone 10g, yeast extract 5g, distilled water 1000ml, pH value 7.4-7.8
[0019] Inorganic phosphorus-solubilizing functional bacterium YH8 was treated with 10 5 The cfu / ml concentration was inoculated in the salinity gradient medium, and each gradient was set up three times. After inoculation, it was shaken and cultured at 28°C for 10 days, and the growth of the strain was recorded. It can grow under the culture conditions of 4.0, 5.0, 6.0, ...
Embodiment 3
[0024] Example 3 Determination of Phosphorus Solubilizing Ability of Inorganic Phosphorus Solubilizing Functional Bacteria YH8
[0025] 1. Preparation of inorganic phosphorus-dissolving functional bacteria YH8 phosphorus-dissolving fermentation broth
[0026] This implementation case is divided into experimental group and blank control group.
[0027] Experimental group: Inorganic phosphorus-solubilizing functional bacteria YH8 (Kushneria sp.) was treated with 10 5 The concentration of cfu / ml was inoculated in a 250ml Erlenmeyer flask equipped with 100ml of inorganic phosphorus bacterial phosphorus-solubilizing liquid medium, and three replicates were set up, and 28°C, 150rpm / m shaking culture for 10 days was obtained to obtain phosphorus-solubilizing fermentation broth. The pH value of the fermentation broth was measured with an Orion MODEL818 pH meter.
[0028] Blank control group: the control group was not inoculated with inorganic phosphorus-dissolving functional bacteri...
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