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Application of hydrogen peroxide in preparing Microcystin inhibitor

A technology of microcystin and hydrogen peroxide, which can be used in applications, disinfectants, biocides, etc., and can solve problems such as secondary pollution

Active Publication Date: 2013-06-19
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, widely used algae-killing drugs include copper sulfate and herbicides, which have a certain algae-killing effect, but copper sulfate and chemical herbicides will have a relatively large negative impact on non-target organisms in the aquatic ecological chain and cause secondary pollution, so caution is required apply

Method used

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  • Application of hydrogen peroxide in preparing Microcystin inhibitor
  • Application of hydrogen peroxide in preparing Microcystin inhibitor
  • Application of hydrogen peroxide in preparing Microcystin inhibitor

Examples

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Embodiment 1

[0017] 1 Culture of Microcystis aeruginosa

[0018] 1) Microcystis aeruginosa was purchased from Wuhan Institute of Hydrobiology, Chinese Academy of Sciences (No. 905), and cultured in BG-11 medium.

[0019] 2) Culture conditions: 24±0.5°C, light-to-dark ratio of 14:10, light intensity: 4000 Lux.

[0020] 3) Dosing: the algae liquid was cultured to OD=0.05, hydrogen peroxide was added at 0, 25, and 50 μM respectively, and samples were taken after culturing for 96 hours.

[0021] 2 Determination of microcystin content

[0022] 1) Take 30ml of algae liquid, centrifuge to remove the supernatant, boil in boiling water for 8min; add 200μl of 75% methanol to mix, centrifuge at 14000rpm for 20min; take the supernatant for determination of intracellular microcystin content.

[0023] 2) Take 30ml of algae liquid, centrifuge to get supernatant, evaporate and concentrate to 10ml; centrifuge at 14000rpm for 20min; take supernatant for determination of extracellular microcystin content. ...

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Abstract

The invention provides application of hydrogen peroxide in preparing a Microcystin inhibitor. The experiment proves that hydrogen peroxide can inhibit synthesis and release of toxins in Microcystis aeruginosa cells, and prevent Microcystin from loss and from entering the water body system. In view of the advantages of high degradability of the Microcystin, the hydrogen peroxide can have wide application prospects in control of water bloom.

Description

(1) Technical field [0001] The invention relates to a new application of hydrogen peroxide in the preparation of microcystin inhibitors. (2) Background technology [0002] The eutrophication of water areas has become a global problem. The eutrophication of freshwater lakes leads to the explosive growth of algae and the formation of algae blooms. At present, 53%, 28%, 48%, and 41% of the freshwater lakes in Europe, Africa, North America, and South America are in eutrophic state to varying degrees, and 54% of the lakes in the Asia-Pacific region are in eutrophic state. The frequency and severity of algal blooms in major freshwater lakes are increasing. More than 75% of my country's freshwater lakes are eutrophic, and large-scale water blooms have frequently occurred in recent years, such as the cyanobacteria outbreaks in Chaohu Lake in August 2003 and June 2006, Taihu Lake and Dianchi Lake in May 2007 bloom. [0003] Blooms are toxic to other organisms by releasing secondary...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N59/00A01P1/00C02F1/50C02F1/00
Inventor 钱海丰余澍琼傅正伟
Owner ZHEJIANG UNIV OF TECH
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