Preparation method of nano magnetic particles for detection and treatment of coronary heart diseases

A technology of nano-magnetic particles and coronary heart disease, which is applied in preparations for in vivo experiments, cardiovascular system diseases, medical preparations of non-active ingredients, etc., can solve the problem of affecting the effect of vascular stenting and difficult to confirm the endothelial cell layer , vascular endothelial cell layer damage, etc., to achieve the effects of prolonging the detection and treatment time, increasing the intensity of magnetic response imaging, and improving sensitivity and reliability

Inactive Publication Date: 2012-09-05
CHENGDU SOUTHWEST JIAOTONG UNIV SCI & TECH GARDEN MANAGEMENT +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are few methods for early diagnosis of coronary heart disease, mainly because it is difficult to identify the endothelial cell layer of the disease
Patients with middle and advanced coronary heart disease are currently mainly treated with drug-eluting vascular stents. Although this treatment can effectively alleviate the disease in the short term, it leads to more serious damage to the vascular endothelial cell layer at the site of stent placement, and then in a period of time after surgery. Intra-stent thrombosis and restenosis are caused, which seriously affects the effect of vascular stenting treatment

Method used

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  • Preparation method of nano magnetic particles for detection and treatment of coronary heart diseases
  • Preparation method of nano magnetic particles for detection and treatment of coronary heart diseases
  • Preparation method of nano magnetic particles for detection and treatment of coronary heart diseases

Examples

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Effect test

Embodiment 1

[0037] A method for preparing magnetic nanoparticles for detection and treatment of coronary heart disease, comprising the following steps:

[0038] A. Immobilizing transition layer molecules on the surface of the magnetic core, which is a compound containing amino, carboxyl or mercapto groups and groups capable of reacting with hydroxyl groups.

[0039] The magnetic core in this example has a particle size between 5nm and is paramagnetic, and its composition is γ-Fe 2 o 3 particles, the transition layer molecule is dopamine.

[0040] The specific method of fixing the transition layer molecules on the surface of the magnetic core in step A of this example is: uniformly disperse the magnetic core in the aqueous solution system of the transition layer molecules, the mass ratio of the magnetic core to the transition layer molecules is 1:0.05, and the fixation time is 0.5h , magnetically separated and washed with deionized water.

[0041] B. Then immobilize functional molecules...

Embodiment 2-30

[0048] The preparation method of embodiment 2-30 is all identical with the operation of embodiment 1 method, and difference is only: the magnetic core that A, each embodiment selects for use, the mass ratio of transition layer molecule, magnetic nucleus / transition layer molecule and fixed time; B, Mass ratio of functional molecular species and magnetic core / functional molecule / EDC / NHS immobilized transition layer molecules; C, ligand species specifically recognized by endothelial progenitor cells and magnetic core / specificity of immobilized functional molecules The mass ratio of the recognition ligand / EDC / NHS was correspondingly replaced with the species, mass ratio, and time listed in Table 1 below (for ease of comparison, the data of Example 1 are also listed in the table).

[0049] Table 1: The fixed time of the material type, material ratio and transition layer molecule that each step of embodiment 1-30 selects

[0050]

[0051]

[0052]

[0053]

[0054] (Note...

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Abstract

The invention relates to a preparation method of nano magnetic particles for detection and treatment of coronary heart diseases, which comprises the following steps: A, mixing transition layer molecules on the surfaces of magnetic nuclei, wherein the transition layer molecule are compounds which simultaneously contain amino groups, carboxyl groups or hydrosulfide groups and groups capable of reacting with hydroxyl groups; B, on the magnetic nuclei fixed with the transition layer molecules, fixing functional molecules capable of prolonging the residence time of nano particles in blood; and C, on the magnetic nuclei fixed with functional molecules, fixing specific recognition ligands of endothelial progenitor cells or diseased endothelial cells, thus obtaining the nano magnetic particles. The method can be used for preparing magnetic nano particles for early-stage detection and location of diseased endothelial cells of coronary heart diseases, and the detection is sensitive and reliable; the method can be also used for preparing magnetic nano particles for mid-late-stage treatment of coronary heart diseases; and good repairing and treatment effects on coronary heart diseases can be achieved.

Description

technical field [0001] The invention belongs to the field of biomedical polymer materials and nano-biotechnology, and in particular relates to a preparation method of nano-magnetic particles used for detection and treatment of coronary heart disease. Background technique [0002] Coronary heart disease is a frequent cardiovascular disease. The number of patients with coronary heart disease in my country is increasing at a rate of 2-4 million per year, which seriously threatens people's health and safety. The primary cause of coronary heart disease is the lesions of vascular endothelial cells, which lead to the gradual formation of plaques in the blood vessels, that is, atherosclerosis, which indicates that patients will enter the middle and late stages of coronary heart disease; plaques not only cause blood flow disorders, but also block blood vessels in severe cases and cause sudden death of patients . So far, there are few methods for early diagnosis of coronary heart dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/18A61K49/08A61K49/12A61K49/14A61K47/48A61P9/10A61K47/52A61K47/60A61K47/61A61K47/62A61K47/69
Inventor 黄楠陈佳龙王娟李全利曹建军陈卓玥熊开琴孙鸿杨苹冷永祥陈俊英王进
Owner CHENGDU SOUTHWEST JIAOTONG UNIV SCI & TECH GARDEN MANAGEMENT
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