Eukaryon recombinant plasmid and application thereof in improvement of accumulation of tomato fruit pigment

Abstract

The invention discloses an eukaryon recombinant plasmid which comprises gene segments in a nucleotide sequences shown in SEQ IN NO:1 in a sequence table and an eukaryon expression vector, wherein the gene segments are nucleotide sequences positioned from a downstream 783bp to 1253bp of an initiation codon and inserted into the eukaryon expression vector in a positive direction and in a negative direction, and an intron with 150bp exists between the gene segments inserted in the positive direction and the gene segments inserted in the negative direction. A preparation method of the eukaryon recombinant plasmid comprises the steps of: 1, selecting the gene segments from the nucleotide sequences shown in SEQ ID NO:1 in the sequence table and inserting the gene segments into a pSK vector in the positive direction to form an intermediate vector, and inserting the gene segments into the pSK vector containing the gene segments inserted in the positive direction in the negative direction; and2, cutting a segment containing the gene segments on the pSK vector by using a restriction incision enzyme and connecting the segment to the eukaryon expression vector. Experiments indicate that the eukaryon recombinant plasmid can be applied in improvement of pigment accumulation of tomato fruits.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, in particular to a eukaryotic recombinant plasmid and its application in regulating tomato fruit pigment accumulation. Background technique [0002] Lycopene is one of the important antioxidants, which has various physiological functions such as scavenging active oxygen free radicals, promoting intercellular gap junction communication, and preventing and fighting cancer. At present, extensive attempts have been made on the genetic manipulation of structural genes in the lycopene synthesis pathway. The main limitation is that strengthening or blocking the expression of a key gene will cause many negative effects, and the accumulation of the target product will not achieve the expected effect. . For example, in the transgenic research to increase the accumulation of lycopene, a crtI gene encoding phytoene desaturase (phytoene desaturase) in microorganisms was transferred into the tomato ge...

AI Technical Summary

Problems solved by technology

At present, extensive attempts have been made on the genetic manipulation of structural genes in the lycopene synthesis pathway. The main limitation is that strengthening or blocking the expression of a key gene will cause many negative effects, and the accumulation of the target product will not achieve the expected effect.

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