Method for detecting procyanidins content

Abstract

The invention provides a method for detecting the procyanidins content, comprising the following steps of sample treatment, chromogenic reagent preparation and sample detection. In the method, ethanol is used as a solvent, replacing methanol, and a dry powder sample is completely dissolved at higher temperature and under ultrasoud, thereby the disadvantage that the sample is difficult to completely dissolve in high-concentration ethanol is solved. With the regulation of the ethanol concentration of a semi-finished solution, the problems that tedious steps of needing to evaporate the semi-finished solution to dryness firstly and then dissolving the sample with the methanol, reducing degradation of procyanidins in the evaporating process and the like of the traditional vanillin-hydrochloric acid method are solved, detection which needs short time without toxicity and is suitable for carrying out in the production field in real time is carried out on a finished product, a semifinishs product or a raw material containing the procyanidins under normal temperature and pressure, a detection result is accurate, the detection is truly accurate, rapid and simple, and the production efficiency of the product is enhanced. Compared with the traditional method for detecting the procyanidins, the method for detecting the procyanidins content is more rapid, the operation is more simple, the repeatability of the result is good, and the accuracy is high.

Description

technical field [0001] The invention relates to a method for detecting the content of proanthocyanidins in natural substances, medicines, health food, beverages and cosmetics. Background technique [0002] Proanthocyanidins, also known as condensed tannins, are a kind of plant polyphenolic compounds with flavan-3-ol structure, which are polymerized by several catechin compounds, and exist in many plants in nature. Among them, plants such as ginkgo leaves, grape seeds, lingonberries, pine bark, lotus pods, and roses all contain proanthocyanidins. Studies have confirmed that proanthocyanidins have strong antioxidant and free radical scavenging capabilities, and have a better protective effect on cardiovascular and cerebrovascular. [0003] Since proanthocyanidins are a class of components and their complex compounds, and there is a lack of commercially available controls, the current determination methods for proanthocyanidins are mostly colorimetric methods, including acid b...

AI Technical Summary

Problems solved by technology

Other methods are mostly methods for detecting polyphenols. For those products with high flavonoid content, the measured values ​​of these methods will be significantly higher

However, the acidic butanol method and the vanillin method with relatively high specificity also have obvious deficiencies in the determination of ginkgo proanthocyanidins: the acidic butanol method is easily affected by anthocyanins and by-products, and the repeatability and reliability of the results are relatively low. Poor; although the sensitivity of the 4-dimethylaminocinnamaldehyde method is higher than that of the vanillin method, because the absorption coefficient of the monomer is significantly higher than that of the polymer, the measurement result is easily affected by the monomer and is obviously higher

But the reaction solvent of the above-mentioned vanillin method is methanol, which is highly toxic, and it is extremely inconvenient to be used for on-line production and detection of products, and needs to be carried out in a special laboratory environment; and these several methods have a long detection time, and the reaction time is all within For more than half an hour, rapid detection cannot be achieved. Since online detection pays attention to speed and accuracy, none of these methods can meet the needs of online detection at present.

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