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Application of resveratrol in preparing medicines for preventing and treating radiation-induced bone marrow suppression

A technology of bone marrow suppression and resveratrol, applied in the preparation of drugs for the prevention and treatment of radiation-induced bone marrow suppression, the application field of polyphenolic compounds in bone marrow suppression drugs

Inactive Publication Date: 2011-08-17
INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature report on the protective effect of Res on ionizing radiation-induced hematopoietic system damage

Method used

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  • Application of resveratrol in preparing medicines for preventing and treating radiation-induced bone marrow suppression
  • Application of resveratrol in preparing medicines for preventing and treating radiation-induced bone marrow suppression
  • Application of resveratrol in preparing medicines for preventing and treating radiation-induced bone marrow suppression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Aseptically take the femur of C57BL / 6 mice (Institute of Experimental Animals, Chinese Academy of Medical Sciences), wash the bone marrow with Hanks solution containing 2% fetal bovine serum, separate by density gradient centrifugation, prepare bone marrow mononuclear cell suspension, wash with PBS, and count Adjust the desired cell concentration before use.

[0032] (2) Determination of cell viability

[0033] Add 100 μl of mononuclear cell suspension to the set wells of the 96-well plate, add 100 μl of treatment drugs according to the design, place them in a 37oC carbon dioxide incubator, and cultivate for 18 hours. Take out the culture plate, put it at room temperature, add 20μl of bioluminescent reagent cell-titer, shake and mix well, then transfer to a black assay plate, GloMax TM The luminescence detector uses Promega's own detection program Cell-titer Protocol for detection. The test results automatically generate Excel data.

[0034] (3) Determination of colo...

Embodiment 2

[0043] (1) Immunocytotoxicity to hematopoietic cells

[0044] Isolate mouse bone marrow mononuclear cells, adjust the cell concentration to 2×10 6 / ml. Different concentrations of Res medium were prepared. 100 μl of cell suspension and different concentrations of treatment drugs were added to 96-well culture plates, and after incubation for 18 hours, cell viability was measured by bioluminescence. As a result, it was found that using RPMI-1640 medium (source? The ingredients? Commercially available?) for experiments, Res at a concentration lower than 10 -4 No obvious cytotoxic effect was found at M. See Table 1 and Table 2 for the results. .

[0045]

[0046] (2) Protective effect on radiation injury of hematopoietic immune cells in vitro

[0047] Bone marrow mononuclear cells were isolated, added different concentrations of drugs, incubated for 30 min, and then irradiated. Continue to incubate for 18h after irradiation, and measure cell viability. The results showe...

Embodiment 3

[0067] Example 4

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Abstract

The invention provides application of a polyphenol compound, such as resveratrol, which is wide in sources, safe and effective, and economic and applicable, in preparing medicines for preventing and treating radiation-induced bone marrow suppression. Results show that the resveratrol has remarkable protection effects on radiation-induced bone marrow cell injuries, such as peripheral blood leukocyte reduction, hypoplastic bone marrow, aplastic anemia and function reduction, and the resveratrol can be used in large dose and has no remarkable toxic and side effects.

Description

technical field [0001] The invention belongs to the technical field of drug application and relates to the application of a known polyphenol compound in bone marrow suppressing drugs. More specifically, it is the application of resveratrol in the preparation of drugs for preventing and treating radiation-induced myelosuppression. Background technique [0002] The body is exposed to various harmful factors produced by external physical chemistry and internal metabolism, such as exposure to radiation or certain chemical poisons, as well as patients receiving radiotherapy and chemotherapy. The composition of different series of hematopoietic cells in the development period is very sensitive to various harmful factors, and will be inhibited to varying degrees after being damaged, manifested as a decrease in peripheral blood leukocytes, a decrease in bone marrow proliferation, and even aplastic anemia, which will affect the quality of life of patients. cause serious harm or even...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61P7/06A61P7/00A61P19/00A23L1/29A23L33/00
Inventor 张恒王月英翟志斌孟爱民侯琦李德冠张俊玲常建辉吴红英王小春路璐
Owner INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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