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Freeze-drying composition containing cerebroprotein hydrolysates and preparation method of freeze-drying composition

A protein hydrolyzate and composition technology, applied in the field of freeze-dried composition containing porcine brain protein hydrolyzate and its preparation, can solve the problems of difficult long-term storage and poor stability, and achieve the effect suitable for clinical use

Inactive Publication Date: 2011-08-31
罗诚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a freeze-dried composition containing porcine cerebroprotein hydrolyzate with good stability and long storage time for the problems of poor stability and long-term storage in existing cerebroprotein injections

Method used

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  • Freeze-drying composition containing cerebroprotein hydrolysates and preparation method of freeze-drying composition
  • Freeze-drying composition containing cerebroprotein hydrolysates and preparation method of freeze-drying composition
  • Freeze-drying composition containing cerebroprotein hydrolysates and preparation method of freeze-drying composition

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Embodiment 1

[0017] 1. Formula (weight: mg): pig brain protein hydrolyzate 250, mannitol 10, reduced glutathione 1, sodium hydroxide 5.

[0018] 2. The preparation method of pig brain protein hydrolyzate:

[0019] 1) Wash the frozen pig brain with water for injection 2-3 times, thaw naturally, add an equal weight of water for injection to the pig brain, and homogenate; 2) Put the homogenate in a hydrolysis tank, and adjust the pH value to 4 with HCl , heated to 45°C, added 10% pepsin according to the weight of the homogenate, after enzymolysis for 20 hours, boiled for 30 minutes, cooled to 30°C to obtain hydrolyzate A; 3) NaOH adjusted the pH value of hydrolyzate A to 8, press Add 10% trypsin to hydrolyzate A by weight, enzymolysis temperature is 45°C, after enzymolysis for 5 hours, boil for 30min, cool to 45°C, filter to obtain crude filtrate B; 4) Add 1% by weight to crude filtrate B Heat and boil medicinal activated carbon, stir and absorb for 20 minutes, cool to room temperature, filt...

Embodiment 2

[0025] 1. Formula (weight: mg): pig brain protein hydrolyzate 600, mannitol 100, reduced glutathione 5.5 and sodium hydroxide 8.

[0026] 2. The preparation method of pig brain protein hydrolyzate:

[0027] 1) Wash the frozen pig brain (derived from healthy animals) with water for injection 2-3 times, thaw naturally, add an equal weight of water for injection to the pig brain, and perform homogenization; 2) Put the homogenate in a hydrolysis tank, Use HCl to adjust the pH value to 5, heat to 50°C, add 12% pepsin according to the weight of the homogenate, after 18 hours of enzymolysis, boil for 30 minutes, cool to 40°C to obtain hydrolyzate A; 3) adjust hydrolyzate A with NaOH The pH value of the solution is 8.5, add 8% trypsin according to the weight of hydrolyzate A, enzymolysis temperature is 50°C, after enzymolysis for 6 hours, boil for 30min, cool to 40°C, filter to obtain crude filtrate B; 4) Add crude filtrate B to According to its weight 0.8%, add medicinal active carb...

Embodiment 3

[0033] 1. Formula (weight: mg): pig brain protein hydrolyzate 350, mannitol 35, reduced glutathione 1.5 and sodium hydroxide 6.

[0034] 2. The preparation method of pig brain protein hydrolyzate:

[0035] 1) Wash the frozen pig brain (derived from healthy animals) with water for injection 2-3 times, thaw naturally, add an equal weight of water for injection to the pig brain, and perform homogenization; 2) Put the homogenate in a hydrolysis tank, Use HCl to adjust the pH value to 4, heat to 45°C, add 10% pepsin according to the weight of the homogenate, after 20 hours of enzymolysis, boil for 30 minutes, cool to 35°C to obtain hydrolyzate A; 3) adjust hydrolyzate A with NaOH The pH value of the solution is 8, add 10% trypsin according to the weight of the hydrolyzate A, enzymolysis temperature is 50°C, after enzymolysis for 5 hours, boil for 30min, cool to 45°C, filter to obtain the crude filtrate B; According to 1% of its weight, add medicinal activated carbon and heat to bo...

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Abstract

The invention provides a freeze-drying composition containing cerebroprotein hydrolysates and a preparation method of the freeze-drying composition. The freeze-drying composition containing the cerebroprotein hydrolysates consists of cerebroprotein hydrolysates, mannitol, sodium hydroxide and reduced type glutathione. The freeze-drying composition containing cerebroprotein hydrolysates can overcome the defects in the conventional cerebroprotein injection that the stability is poor and polypeptide matters are easily subject to environmental influence and generate polymerization, and are more suitable for clinical application.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations, in particular to a freeze-dried composition containing porcine brain protein hydrolyzate and a preparation method thereof. Background technique [0002] Traumatic brain injury and sequelae of cerebrovascular disease seriously threaten human health. Cerebroprotein hydrolyzate contains biologically active low-molecular-weight polypeptides and 16 kinds of amino acids necessary for the human body, which is conducive to the synthesis of intracellular proteins and effectively protects the central nervous system from damage. Improve the anti-hypoxic ability of the brain and improve the blood supply of cells. [0003] Studies have shown that cerebroprotein injection has poor stability. During long-term storage and long-distance transportation, polypeptides are susceptible to aggregation due to environmental influences, which affects product quality, and clinical allergic reactions are prone to ...

Claims

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Application Information

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IPC IPC(8): A61K9/19A61K38/01A61K35/30A61K47/42C12P21/06A61P9/10A61P25/00A61K47/18
Inventor 罗诚
Owner 罗诚
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