Device for protein separation and purification teaching experiment

Abstract

The invention discloses a device for a protein separation and purification teaching experiment, which comprises a perfusion container, wherein a stirrer is arranged in the perfusion container; and the bottom of the container is provided with a drainage tube. The device is characterized in that a rotating disk is arranged below the perfusion container, and a plurality of chromatographic column test tubes are arranged on the circumference of the rotating disc; when each chromatographic column test tube is static, an opening at the upper end is over against an outlet of the drainage tube; the side wall of the drainage tube is provided with a switch plug; the switch plug and the rotating disk realize linkage by a flow control and steering device, i.e. when the switch plug closes the drainage tube, the rotating disk rotates, the chromatographic column test tube which is over against the outlet of the drainage tube is separated, and the adjacent chromatographic column test tube moves to theoutlet of the drainage tube; when the switch plug opens the drainage tube, the rotating disk stops, and the chromatographic column test tube on the rotating disk is static; and a collecting tank which can receive waste liquid of the chromatographic column test tube is arranged below the rotating disk.

Description

technical field [0001] The invention relates to a teaching experiment device used for protein separation, purification and identification in biochemistry and molecular biology experiments. Background technique [0002] In recent years, the rapid development of modern biochemistry and molecular biology research technology represented by recombinant DNA technology has injected new vigor and vitality into the development of biochemistry and molecular biology theory. There will be important theoretical breakthroughs. Among them, protein separation, purification and identification is a key link, especially in the process of experimental teaching, which can not only stimulate students' learning enthusiasm and curiosity, but also broaden students' horizons. However, in the teaching experiment process, protein separation and purification is particularly complicated and cumbersome, and the operation is extremely inconvenient. In the past, the teaching and experimental equipment for...

AI Technical Summary

Problems solved by technology

However, in the teaching experiment process, protein separation and purification is particularly complicated and cumbersome, and the operation is extremely inconvenient

In the past, the teaching and experimental equipment for protein separation and purification was relatively simple and time-consuming. The entire production process was manual. The specific operation process was to continuously heat and stir the dextran and cellulose, and finally made the semi-finished carrier At this time, it is easy to appear difficult to dissolve, which brings a lot of inconvenience to scientific research and teaching; then slowly pour it into the chromatography column with stirring, and it is required that neither overflow nor air bubbles enter the chromatography column, so repeatedly 4-5 times to make a chromatographic column

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