Unlock instant, AI-driven research and patent intelligence for your innovation.
Method for screening anti-aids drug
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A screening method and anti-AIDS technology, applied in the field of molecular biology, can solve problems such as cost constraints and achieve accurate drug effects
Inactive Publication Date: 2013-05-08
ZHEJIANG UNIV
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Because HIV is infectious to humans, it must be strictly limited to negative pressure P3 laboratories, and the high cost restricts large-scale general screening of drugs
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0031] Embodiment 1, the selection of optimal cell ratio and optimal co-culture conditions
[0032] (1) Normal cell culture (that is, the cells can grow normally) JLTRG cells and H9 cells are cultured using 1640 medium containing 10% FBS, 1% streptomycin and 1% penicillin (that is, adding 100g per liter of 1640 medium FBS of 10g, streptomycin of 10g and penicillin of 10g), cell count; Generally, the number of suspension in every 2ml of 1640 culture medium containing 10% FBS, 1% streptomycin and 1% penicillin is 10 6 Cell. Cultured in a cell cultureincubator at 37°C with CO 2 The concentration (volume concentration) is 5%, and the humidity (relative humidity) is 75%. The incubation time is generally 24 hours.
[0033] (2) Put JLTRG cells and H9 cells in 5% FBS, 1% streptomycin and 1% Penicillin 1640 medium co-cultured for 24 hours, 48 hours, 72 hours, 96 hours. Cultured in a cell cultureincubator at 37°C with CO 2 The concentration is 5% and the humidity is 75%. The nu...
Embodiment 2
[0039] Embodiment 2, take three kinds of existing medicines (T20, EFV, PTD) as experimental detection target, determine the practicability of the method of the present invention:
[0040] According to the experimental conditions screened above, JLTRG cells and H9 cells were co-cultured in 1640 medium with 5% FBS, 1% streptomycin and 1% penicillin at a cell ratio of 10:1.
[0045] (2) Cultivate the above-mentioned three types of drug solutions with different concentrations and JLTRG cells for 6 hours. The number of each hole is 10 6 The JLTRG cells were cultivated in a cell cultureincubator at 37°C, and the CO in the cell culture incubator 2 The concentration is 5%, t...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a method for screening an anti-aids drug. The method comprises the following steps: 1) diluting the drug to be detected into drug solutions of different concentrations; 2) nurturing JLTRG cells in the drug solutions; 3) adding a 1640 culture medium containing 5% FBS, 1% streptomycin and 1% penicillin as well as H9 cells to perform co-culture; 4) measuring the expression condition of green fluorescence with a flow cytometer, and obtaining the corrected cellfluorescence value according to the percentage of the green fluorescence cells and the average fluorescence intensity; 5) separately setting positive control and negative control; and 6) measuring the effective dose resisting half of aids, of the drug to be measured according to the experiment result. The method disclosed by the invention can quickly and effectively implement screening of the anti-aids drug.
Description
technical field [0001] The invention belongs to the technical field of molecular biology, and relates to an anti-AIDS drug screening technology and a research method. Background technique [0002] AIDS (AIDS) is a kind of human immunodeficiencyvirus (human immunodeficiencyvirus, referred to as HIV) that invades the human body and destroys the immune function of the human body, causing a variety of incurable infections and tumors in the human body, and finally leading to the death of the infected person. a serious infectious disease. Looking back over the past 25 years, the prevalence of AIDS has far exceeded people's fears and estimates. Until now, the medical community has not yet found the best solution to this disease. Relevant scientific research institutions are trying to find the weakness in the virus's own defense, so as to develop antiviral drugs targeting the virus itself and host factors in order to inhibit or kill the virus. the virus. [0003] The rapid rise...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
