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Application of combined quantitative detection of IL-1[beta], IL-6, IL-10, IL-17, IL-23 and TNF-[alpha] in serum in early-stage prediction of liver cancer

A technology of IL-23 and IL-6, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problem of lack of literature reports on the value of liver cancer diagnosis and early warning

Inactive Publication Date: 2014-07-02
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] However, the role of these substances in the occurrence of liver cancer and their value in the diagnosis and early warning of liver cancer have not been reported in the literature.

Method used

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  • Application of combined quantitative detection of IL-1[beta], IL-6, IL-10, IL-17, IL-23 and TNF-[alpha] in serum in early-stage prediction of liver cancer
  • Application of combined quantitative detection of IL-1[beta], IL-6, IL-10, IL-17, IL-23 and TNF-[alpha] in serum in early-stage prediction of liver cancer
  • Application of combined quantitative detection of IL-1[beta], IL-6, IL-10, IL-17, IL-23 and TNF-[alpha] in serum in early-stage prediction of liver cancer

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Experimental program
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Embodiment 1

[0110]From 1996 to 1999, in Qidong City, Jiangsu Province, patients with chronic HBV infection who tested positive for HBV surface antigen at least twice within 2 years were recruited to establish a follow-up cohort of chronic HBV infected patients. Patients with hepatitis (HCV) virus or human immunodeficiency virus (HIV) co-infection and other concomitant chronic liver diseases were excluded from this study, and those with liver cirrhosis or hepatocellular carcinoma detected by B ultrasound at the time of enrollment were also excluded In the analysis of this study, the cohort population had not received formal antiviral treatment. The study protocol was approved by the Ethics Committee of Cancer Institute / Hospital, Chinese Academy of Medical Sciences and Qidong Liver Cancer Institute, Chinese Academy of Medical Sciences and Qidong Liver Cancer Institute (approval number: #09-59 / 354 ).

[0111] All patients who progressed to liver cancer were diagnosed according to the "Clini...

Embodiment 2

[0113] Embodiment 2: experiment and statistical method

[0114] Quansys (Quansys Biosciences, Logan, Utah) Human 9-plex assay kit was used to detect the levels of inflammatory cytokines in patient serum, which contained the following nine cytokines: IL-1α, IL-1β, IL- 6. IL-8, IL-10, IL-17, IL-23, IFNγ and TNFα. The experimental procedure was carried out according to the guidance of the manufacturer's manual, and the detection range of this analysis was as follows:

[0115] IL-1α: 0.87-450pg / ml;

[0116] IL-1β: 3.24-1400pg / ml;

[0117] IL-6: 3.81-475pg / ml;

[0118] IL-8: 0.21-125pg / ml;

[0119] IL-10: 0.22-190pg / ml;

[0120] IL-17: 1.63-450pg / ml;

[0121] IL-23: 18.64-8000pg / ml;

[0122] TNFα: 0.73-350pg / ml;

[0123] IFNγ: 0.25-100 pg / ml.

[0124] Cytokine concentrations outside the detection range were estimated by the software. Each sample was tested in duplicate, and the average value was used for data analysis. To assess the effect of storage time on cytokine sta...

Embodiment 3

[0126] Embodiment 3: experimental result

[0127] As of June 2009, 47 cases of chronic HBV infection who met the above analysis conditions had progressed to liver cancer. According to the ratio of 1:3, in the same research cohort, 141 cases matched in age and sex were selected and undiagnosed Liver cancer patients were used as controls for nested case-control study analysis. For patients who developed from chronic HBV infection to liver cancer, the follow-up time (expressed as the median (25%-75%) quantile) was 108 (84-132) months, and the chronic HBV infection group that was not diagnosed as liver cancer was 144 (132-144) months (p<0.001). Some characteristics between the HCC group (patients who progressed from chronic HBV infection to HCC) and the control group (patients who maintained chronic HBV infection status without being diagnosed with HCC) are as follows (Table 2). The median age of both the liver cancer group and the control group at enrollment was 44 years old (p...

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Abstract

The invention relates to a method for identifying tendency for liver cancer of hepatitis virus-infected subjects. The method comprises detecting levels of at least two factors of IL-1[beta], IL-6, IL-10, IL-17, IL-23 and TNF-[alpha] contained in a biological sample of the subjects, wherein increment of the level is an indication of tendency for liver cancer of the subjects. The invention also relates to application of substances for detecting at least two factors of IL-1[beta], IL-6, IL-10, IL-17, IL-23 and TNF-[alpha] in preparation of compositions for identifying tendency for liver cancer of a subject and in a kit for identification.

Description

field of invention [0001] The invention relates to a new marker and method for early warning diagnosis of liver cancer, and also relates to a kit for early warning diagnosis of liver cancer. Background of the invention [0002] Primary liver cancer is one of the malignant tumors with the highest incidence rate in my country. The number of deaths from liver cancer accounts for about 50% of the world's liver cancer deaths every year, causing a heavy economic burden to the patient's family and the country. In my country, chronic hepatitis B virus (HBV) infection is the main cause of liver cancer, manifested as persistent positive serum HBV surface antigen. According to the epidemiological survey results in 2006, the positive rate of HBV surface antigen among residents aged 1-59 in my country is as high as 7.18%, and there are approximately 93 million HBV surface antigen carriers. [0003] Liver cancer progresses rapidly, is not sensitive to chemotherapy, and has a high rate of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/576
Inventor 曲春枫杜君陈陶阳王金兵
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI