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Genetic incorporation of 3-aminotyrosine into reductases

A technology of reductase and tyrosine, applied in the field of protein chemistry, can solve the problem of no activity hindering

Inactive Publication Date: 2011-09-28
THE SCRIPPS RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, unlike residue Y in β2 356 Similarly, these mutants (Y 730 F-α2 and Y 731 F-α2) inactivity hampers study Y 730 and Y 731 role in the spread of free radicals

Method used

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  • Genetic incorporation of 3-aminotyrosine into reductases
  • Genetic incorporation of 3-aminotyrosine into reductases
  • Genetic incorporation of 3-aminotyrosine into reductases

Examples

Experimental program
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Effect test

Embodiment 1

[0076] Example 1: Site-specific insertion of 3-aminotyrosine into subunit α2 of E. coli ribonucleotide reductase: Y 730 and Y 731 Direct evidence of involvement in the spread of free radicals

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PUM

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Abstract

This invention provides reductase proteins that comprise NH2Y unnatural amino acid residues, systems of orthogonal elements for incorporating NH2Y into reductases and methods of using NH2Y amino acid residues in reductases as molecular probes for probing reductases function, structure and activity.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application Serial No. 61 / 000,491, filed August 25, 2007, and Provisional Patent Application Serial No. 61 / 001,265, filed August 30, 2007, the contents of which are incorporated by reference in their entirety for all purposes incorporated into this article. [0003] R&D under funding [0004] This invention was made with government support under National Institutes of Health Grant No. 5R01 GM62159 and Grant No. GM29595. The government has certain rights in this invention. field of invention [0005] The present invention relates to the fields of protein chemistry, such as translational biochemistry and mutation analysis. The present invention relates to methods and compositions for producing reductase enzymes comprising 3-aminotyrosine residues and for determining the function of selected amino acid residues in the reductase enzymes. Background of the inven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N5/10C12Q1/26G01N33/573
CPCC12Q1/26C12N9/0093G01N2333/9029
Inventor M·R·塞德萨姆多斯特谢建明C·T·常L·阿方达P·G·舒尔茨J·斯多比
Owner THE SCRIPPS RES INST