Microfluidic multiplexed cellular and molecular analysis device and method

Abstract

A sequential flow analysis tool comprising a microti iridic device having a fluid path defined within a substrate between an input and an output is described. The device includes a capture chamber provided within but offset from the fluid path, the capture chamber extending into the substrate in a direction substantially perpendicular to the fluid path such that operably particles provided within a fluid flowing within the fluid path will preferentially collect within the capture chamber.

Description

field of invention [0001] The present invention relates to microfluidic devices and assays using the devices. The present invention more particularly relates to microfluidic devices and methods useful for multiplexed cellular and molecular analysis and processing. Background technique [0002] Microfluidic devices are well known for sample processing and analysis. Such devices provide precise control and manipulation of fluids and are generally considered to have geometries on the micron or submillimeter scale. These devices are particularly useful because they can provide measurements where only small volumes of analyte are available or where small amounts of reagents should be used eg in high throughput screening for drug discovery. Furthermore, they readily provide results with reduced reagent consumption and analysis time, facilitating integration and potential for high-throughput analysis. [0003] While conventional microfluidic devices offer many advantages commens...

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Problems solved by technology

Moreover, manufacturing costs must be minimized, limiting the range of manufacturing techniques and thus the degrees of freedom available for device feature geometries

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