Method for cultivating polyploid dianthus caryophyllus germplasm

A cultivation method and technology of carnation, applied in the field of plant genetics and breeding, can solve the problems of narrow genetic background, degeneration, incompatibility and the like of carnation, so as to overcome the decline of hybrid fertility and viability, stable traits and extensive genetic variation. Effect

Inactive Publication Date: 2013-04-10
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems that Carnation has a narrow genetic background, most germplasm resources are diploid, and incompatibility before fertilization occurs in the hybridization process of different ploidy Carnation varieties, resulting in abortion or degeneration of embryos, the present invention provides a method using 2n Gamete and Embryo Culture Technology Innovation Method for Polyploid Carnation Germplasm Resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A. The multi-head carnation germplasm resource "Purple Butterfly" (2n=4x=60) was selected as the female parent. Its flower color and shape are unique, the growth period is short, and the tissue culture seedlings have strong reproductive ability, high reproduction coefficient, but low fertility and resistance. It has weak disease resistance and is susceptible to Fusarium wilt; the single-petal selection line NH10 (2n=2x=30) is used as the male parent, which has high fertility, high yield and strong disease resistance. The frequency of 2n gametes production is greater than 0.01% , In the blooming period, the female parent is emasculated and bagged. 10 days after bagging, the stigma of the female parent is bent and the top is curled, and when the stigma is covered with fluff, the male parent pollen that has just loosened is collected. At this time, the amount of pollen is large and the pollen High vigor, carry out artificial pollination, go to the bag and strip the ovary af...

Embodiment 2

[0021] A. The multi-head carnation germplasm resource "Purple Butterfly" (2n=4x=60) was selected as the female parent. Its flower color and shape are unique, the growth period is short, and the tissue culture seedlings have strong reproductive ability, high reproduction coefficient, but low fertility and resistance. It has weak disease resistance and is susceptible to fusarium wilt; the single-petal selection line NH10 (2n=2x=30) is used as the male parent, which has the characteristics of high fertility, high yield and strong disease resistance. The frequency of 2n gametes is greater than 0.01%. In the blooming period, the female parent is emasculated and bagged. After 12 days of bagging, the stigma of the female parent is bent and the top is curled, and the stigma is covered with fluff. Collect the pollen of the male parent that has just dispersed. Large, high pollen vigor, for artificial pollination, 21 days after pollination, go to the bag and peel the ovary

[0022] B. The...

Embodiment 3

[0027] A. The multi-head carnation germplasm resource "Purple Butterfly" (2n=4x=60) was selected as the female parent. Its flower color and shape are unique, the growth period is short, and the tissue culture seedlings have strong reproductive ability, high reproduction coefficient, but low fertility and resistance. It is weakly diseased and is susceptible to Fusarium wilt; the single-petal selection line NH14 (2n=2x=30) is used as the male parent, which has the characteristics of high fertility, high yield and strong disease resistance. The frequency of 2n gametes is greater than 0.01%. In the blooming period, the female parent is emasculated and bagged. After 7 days of bagging, the stigma of the female parent is bent and the top is curled, and the stigma is covered with fluff. Collect the pollen of the male parent that has just dispersed. Large, high pollen vigor, carry out artificial pollination, and peel off the ovary after 45 days of pollination;

[0028] B. The ovary stri...

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PUM

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Abstract

The invention discloses a method for cultivating polyploid dianthus caryophyllus germ plasm, which comprises the following steps: 2n gamete-containing diploid germplasm is cultivated which can be taken as a male parent to hybridize with a tetraploid female parent, the female parent is performed a castration and a bagging for 7 to 14 days during a full bloom stage, then an artificial pollination is performed, and the bag is removed and an ovary is taken off after pollinating for 15 - 45 days; the ovary is carried out a disinfection treatment and then cut off for taking off a white ovule, the ovule is performed an inoculation on an embryo culture medium for cultivating, the white ovule is grown to a black seed, a seed coat is cut off and a white embryo is taken off for continuous cultivating on a embryo culture medium, then a cultivation is conducted on a successive transfer culture medium for obtaining the polyploid dianthus caryophyllus germplasm. According to the invention, the diploid favorable gene and heterozygosity are successfully introduced in a tetraploid cultivation, which overcomes a problem of embryo abortion and greatly enriches a gene database of carnation genetic breeding, the invention is an effective approach of an objective trait introgression, and is beneficial to germplasmic introgression and gene flow of different ploidies, the hybridized progeny has more widely genetic variation and stable property as well.

Description

Technical field [0001] The invention relates to a breeding method for polyploid plants, in particular to a method for cultivating polyploid carnation germplasm by using 2n gametes and embryo culture, and belongs to the technical field of plant genetic breeding. Background technique [0002] Polyploid plants have important ornamental value due to their large flowers, strong flower colors, and strong stems. In addition, polyploidy is also an important method to overcome contemporary infertility in distant hybridization and lack of fruit in distant hybrids. 2n gamete hybridization is considered to be a very effective way of gene introgression of target traits, which is conducive to the introgression and gene flow of different ploidy germplasm, and the hybrid offspring has a wider range of genetic variation, and the traits of the hybrid offspring are also very stable. Therefore, the use of 2n gametes to create a polyploid hybrid population with high heterozygosity and rich genetic h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 周旭红王继华张颢莫锡君桂敏王慧敏陈敏
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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