Avian infectious bronchitis cold adaptation attenuated vaccine strain and application thereof

A technology for bronchitis and chicken infectiousness, applied in the field of prevention and treatment of chicken infectious bronchitis, can solve the problems of inability to effectively prevent and control the attack of popular strains of chicken infectious bronchitis virus and unsatisfactory control effect, and achieve Good protection effect, good safety effect

Active Publication Date: 2011-10-19
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The main technical problem to be solved by the present invention is to overcome defects such as the inability of existing vaccine strains to effectively prevent the attack of the popular strain of chicken infectious bronchitis virus, and

Method used

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  • Avian infectious bronchitis cold adaptation attenuated vaccine strain and application thereof
  • Avian infectious bronchitis cold adaptation attenuated vaccine strain and application thereof
  • Avian infectious bronchitis cold adaptation attenuated vaccine strain and application thereof

Examples

Experimental program
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Effect test

experiment example 1

[0024] Experimental example 1 Breeding and identification of chicken infectious bronchitis cold-adapted attenuated vaccine strain LDL70-C strain of the present invention

[0025] 1 Materials and methods

[0026] 1.1 The cold-adapted subculture of virus strain LDL091022 was attenuated

[0027] Use 9-10 day-old SPF chicken embryos to carry out continuous low-temperature cold-adapted subculture of the highly virulent IBV strain LDL091022. In each passage, 3 chicken embryos were inoculated through the allantoic cavity, and each embryo was inoculated with 100 μL. The culture conditions were as follows: from 35°C At the beginning, reduce 1°C every 10 generations, and incubate for 72 hours for each generation. After 72 hours, take out the chicken embryos, observe the lesions of the chicken embryos, collect the allantoic fluid of the chicken embryos aseptically, and carry out the next passage until the incubation temperature reaches 30°C. The later subcultures were all cultured at 30...

experiment example 2

[0058] Experimental example 2 The immune efficacy and protective effect experiment of the cold-adapted attenuated vaccine strain LDL70-C strain of the present invention

[0059] 1. Experimental method

[0060] 1.1 Preliminary evaluation of the immune efficacy of LDL70-C

[0061] 1.1.1 Immunological efficacy test of 10-day-old SPF chicken

[0062] Forty 1-day-old SPF chickens were randomly divided into 2 groups (20 in each group), and were raised in a negative pressure isolator, and the chicks had free access to food and water. At 10 days of age, one of the group of chicks was treated with LDL70-C (P70, 10 4 EID 50 ) for inoculation with 100 μL intranasally; another group of chicks was used as a control group with 100 μL normal allantoic fluid intranasally. From the date of inoculation, observe and record the incidence and death of inoculated chickens every day, and 14 days after immunization, collect serum from each group for specific antibody detection. For specific metho...

experiment example 3

[0076] Experimental example 3 Preparation of chicken infectious bronchitis attenuated vaccine

[0077] Take the attenuated vaccine strain of the present invention (LDL70-C strain), restore the culture condition to 37°C, proliferate the virus seeds for production, dilute the virus seeds for production by 100,000 times with sterilized physiological saline, and inoculate them in 9-10 day-old SPF chicken embryos In the allantoic cavity, inoculate 0.1ml per embryo, seal the pinhole after inoculation, and incubate at 37°C. The chicken embryos were inoculated for 24 hours and the embryos were illuminated, the dead embryos were discarded, and the air chamber of the chicken embryos incubated at 37°C for 30-72 hours was upward, and placed at 4°C to cool for 4-24 hours. Harvest the virus allantoic fluid, mix several chicken embryo allantoic fluids into a group, put them in a sterilized bottle, store them at 2-8°C, and do a sterility test at the same time, and the virus content should be ...

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Abstract

The invention discloses an avian infectious bronchitis cold adaptation attenuated vaccine strain and application thereof. In the invention, an avian infectious bronchitis virus wild strong strain is subject to continuous low-temperature cold adaption passage attenuated culture so as to obtain a cold adaption weak virus vaccine strain with good immune protective efficiency, and the microbial preservation number is CGMCC No.4711. Immune efficiency evaluation experiments show that the cold adaptation attenuated vaccine strain disclosed by the invention has good immune protectiveness, the active immune protection rate is over 80%, the passive immune can get over 90% protection within 9 days, and the cold adaptation attenuated vaccine strain has good protective efficiency to avian infectious bronchitis, and can effectively protect chicken against the attack of the virulence of avian infectious bronchitis viruses. Safety experiments show that the cold adaption weak virus live vaccine disclosed by the invention is safe for chickens and layer chickens, has no side effects and has good safety. The cold adaption attenuated vaccine strain can be applied in the preparation of single vaccine or combined vaccine and the like for preventing the avian infectious bronchitis viruses.

Description

technical field [0001] The invention relates to an attenuated live vaccine strain, in particular to a cold-adapted attenuated live vaccine strain for chicken infectious bronchitis and an application thereof, belonging to the field of prevention and treatment of chicken infectious bronchitis. Background technique [0002] Chicken infectious bronchitis (Avian Infectious Bronchitis, IB) is a kind of acute, highly contagious, viral respiratory disease caused by chicken infectious bronchitis virus (Infectious Bronchitis Virus, IBV), it is characterized in that: sick chicken coughs , sneezing, tracheal rales; enlarged, pale kidneys, dilated ureters, a large amount of urate deposition, and the appearance of a typical "mottled kidney". [0003] Chicken infectious bronchitis was first discovered in North Dakota, USA in 1930 and first reported by Schalk and Hawn in 1931. Early studies have shown that chicken infectious bronchitis virus mainly affects the respiratory tract of chicks (...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N7/08A61K39/215A61P31/14C12R1/93
Inventor 刘胜旺韩宗玺邵昱昊刘晓丽孔宪刚
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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