Locusta migratoria manilensis locust nymph attractant and preparation method thereof
A technology of attractants and locust gnats, applied in the direction of attracting pests, botanical equipment and methods, biocides, etc., to achieve the effect of control and monitoring population dynamics
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Embodiment 1
[0021] Embodiment 1, East Asia migratory locust locust gnat attractant and its attracting effect to East Asia migratory locust fourth instar locust nymph
[0022] Mix 1ml of paraffin oil with 0.1 μL, 0.5 μL, 1.0 μL, 10 μL, 30 μL, 50 μL and 100 μL of 2,5 dimethylpyrazine at room temperature to obtain the nymph attractant of East Asian migratory locust.
[0023] The Y-type locust olfactory behavior instrument provided by the Chinese invention patent ZL200810056093.4 is used to measure the tendency response of the fourth instar locust gnat of the East Asian migratory locust to the attractant. The specific method is:
[0024] Place the test insects at the entrance of the above-mentioned Y-type locust olfactory behavior instrument, start timing, and each insect takes five minutes. When the test insects enter the A area or B area of the olfactory instrument and are still, it is considered that the test insects have made If the test insect is still in the C area after five minutes,...
Embodiment 2
[0030] Embodiment 2, the attractant of migratory locust migratory locust and its lure to the gnat gnat of migratory locust east asia five instars
[0031] Mix 1ml of paraffin oil with 0.1 μL, 0.5 μL, 1.0 μL, 10 μL, 30 μL, 50 μL and 100 μL of 2,5 dimethylpyrazine at room temperature to obtain the nymph attractant of East Asian migratory locust.
[0032] According to the same method as in Example 1, the tendency response of the fifth-instar locust gnats to the attractant was determined.
[0033] The above-mentioned assay method is set to repeat three times, and the average value is taken for data analysis, and the obtained results are as follows: image 3 and Figure 4 As shown, the ck group in the figure is the control group. in, image 3Represent the lure effect of the migratory locust attractant on five instar migratory locust gnats, wherein the abscissa concentration is the volume ratio of 2,5-dimethylpyrazine and paraffin oil, wherein, 2,5-dimethylpyrazine The volume un...
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