Unlock instant, AI-driven research and patent intelligence for your innovation.
A method for identifying salt-tolerant cotton and special ssr marker
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A cotton and salt-tolerant technology, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the difficulties in breeding and identification of salt-tolerant varieties, complex salt-tolerant cotton, and lack of unity Recognize and other issues, achieve stable and reliable results, not subject to seasonal restrictions, and simple operation
Active Publication Date: 2015-09-30
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0002] The salt tolerance of cotton is a very complex trait, involving a variety of physiological and biochemical metabolic pathways and many gene regulation. There are large differences in salt tolerance between varieties, different growth stages of the same variety, and different tissues and organs. There may be many different mechanisms of salt tolerance. So far, no unified understanding has been formed, and there are still different opinions in many fields and argue
The complexity of salt tolerance of cotton brings great difficulties to the selection and identification of salt-tolerant varieties
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0080] Example 1. Salt tolerance-related marker screening
[0081] 1. Screening of a single marker
[0082] First, 4 materials with different salt tolerance (2 typical salt-tolerant materials Zhong 9835 and Zhong 07, 2 typical salt-sensitive materials Zhong S9612 and Xinyan 96-48) were selected for SSR primer screening ( figure 1 ). 246 pairs of polymorphic primers were initially screened out of 5053 pairs of SSR primers, accounting for 6.21%. These 246 pairs of primers were used as candidate primers for further research.
[0083] Using 6 materials: 9835, Zhong 07, Zhong 9806, Zhong S9612, Xinyan 96-48 and 16 pairs of Lumianyan, 246 pairs of SSR were screened for further re-screening, and 95 pairs with obvious differences, clear and easy to identify band patterns were selected The primers were used for PCR amplification of 48 test materials. Through careful observation and analysis of the electrophoretic bands, it was found that 5 markers (CSHES149, HAU773, HAU1058, HAU191...
Embodiment 2
[0133] Embodiment 2, carrying out salt-tolerant cotton identification with marker combination
[0134] The details of the 11 materials used in this example are as follows: J1: Zhongjian 9901; J2: Zhongjian 9902; J3: Zhongjian 9903; J4: Zhongjian 9904; J5: Zhongjian 9905; J6: Zhongjian 9906; J7: Zhongjian 9907; J8: Zhongjian 9908; J9: Zhongjian 9909; J10: Zhongjian 9910; J11: Zhongjian 9911;
[0135] The public can purchase the above 11 materials from the National Cotton Germplasm Bank of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences.
[0136] (1) Single plant detection method
[0137] 1. Materials: 11 materials (J1~J11).
[0138] 2. Three-marker combination: HAU1918(T4), HAU1058(T3A and T3B) and TMB1288(S1)
[0139] 3. Method:
[0140] The DNA of one individual plant from each material was randomly selected, and then used as a template for SSR-PCR amplification and detection of amplified products.
[0141] According to the individual plant ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a method and special SSR (Simple Sequence Repeat) markers for identifying salt-tolerant cotton. The primer compositions for auxiliarily identifying salt-tolerant cotton are HAU1918, HAU1058 and TMB1288. The experiment proves that the method disclosed by the invention has stable and reliable result, is simple to operate and is economic and efficient. Compared with the morphological identification method, the method disclosed by the invention has the advantages of high efficiency, high accuracy, time saving, labor saving and the like, is free from the restrictions of seasons, has wide application prospects, and can be used for identifying and evaluating salt tolerance of cotton in production practice. Therefore, the SSR marker compositions and method for identifying salt-tolerant cotton can have wide application prospects in the field of salt tolerance identification.
Description
technical field [0001] The invention relates to a method for identifying salt-tolerant cotton and a special SSR marker. Background technique [0002] The salt tolerance of cotton is a very complex trait, involving a variety of physiological and biochemical metabolic pathways and many gene regulation. There are large differences in salt tolerance between varieties, different growth stages of the same variety, and different tissues and organs. There may be many different mechanisms of salt tolerance. So far, no unified understanding has been formed, and there are still different opinions in many fields and argue. The complexity of salt tolerance of cotton brings great difficulties to the selection and identification of salt-tolerant varieties. If several markers related to cotton salt tolerance can be screened, and the salt tolerance of cotton can be directly identified and evaluated at the molecular level, it will be a major breakthrough in the identification method of cott...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More