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Method for detecting 5,7-dyhydroxy chromone, eriodictyol and luteolin in peanut shell simultaneously

A technology of luteolin and dihydroxy, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of improving detection efficiency, good reproducibility, and high precision

Inactive Publication Date: 2013-01-23
山东创新源农业技术开发有限公司
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Problems solved by technology

[0003] 5,7-dihydroxychromanone, eriodictyol and luteolin are the main functional components in peanut shells. In literature reports, only the determination of 5,7-dihydroxychromanone and eriodictyol in peanut shells and the separate determination The method of luteolin, but the method of simultaneous detection of 5,7-dihydroxychromanone, eriodictyol and luteolin by high performance liquid chromatography has not been reported yet

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  • Method for detecting 5,7-dyhydroxy chromone, eriodictyol and luteolin in peanut shell simultaneously

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Embodiment Construction

[0020] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, so that those skilled in the art can understand the present invention.

[0021] Preparation of the control solution: Weigh 2.4mg, 2.4mg and 3.8mg of the three reference substances of 5,7-dihydroxychromanone, eriodictyol and luteolin into a 25ml volumetric flask, add 100% chromatographic methanol to dissolve, Dilute the volume to the mark, and use it as a reference solution for subsequent use;

[0022] Preparation of the solution to be tested: remove impurities from the peanut shells, wash and dry them, crush them with a traditional Chinese medicine grinder, pass through a 60-mesh sieve, accurately weigh 1.000 g of peanut shell powder, add 30 mL of methanol for ultrasonic extraction for 40 minutes, filter and dilute to 50mL, the filtrate is filtered with a 0.45um organic membrane, which is the sample solution to be tested (equivalent to 20mg / mL of...

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Abstract

The invention belongs to the field of food detection, and particularly relates to a method for detecting 5,7-dyhydroxy chromone, eriodictyol and luteolin which serve as main active ingredients in a peanut shell simultaneously. In the method, three active ingredients are detected by high performance liquid chromatography and by using octadecyl silane bonded silica gel as a filling agent and methanol and an aqueous gradient elution solvent as a mobile phase and regulating the pH value by acid; and sample introduction is performed once, and a chromatographic peak of each component is better separated to obtain the detection effect with high accuracy and repeatability. The method for detecting the 5,7-dyhydroxy chromone, the eriodictyol and the luteolin in the peanut shell is simple, easy andconvenient to operate, low in cost and high in efficiency, and is a shortcut for the quality control of the raw materials and products.

Description

technical field [0001] The invention belongs to the field of food detection, and in particular relates to a method capable of simultaneously detecting 5,7-dihydroxychromone, eriodictol and luteolin in peanut shells. Background technique [0002] Peanut (Peanut) is derived from the dried pods of the annual herbaceous plant Peanut (Arachis hypogaea L.) of the leguminous family, and the peanut hull (Peanut hull) is the shell of the pods. my country's peanut shells are rich in resources and low in price, and have broad application prospects in the fields of medicine, industry, agriculture, and health food. Peanut shell is a Chinese herbal medicine in the folk, which can treat asthma, phlegm, high blood pressure and so on. The flavonoids represented by luteolin in peanut shells have various pharmacological activities such as lowering blood pressure, lowering blood fat, dilating coronary arteries, anti-oxidation, antitussive, expectorant and asthma, anti-bacterial and anti-inflam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/14
Inventor 杜方岭裘纪莹祝清俊王文亮陈蕾蕾孙欣刘孝永
Owner 山东创新源农业技术开发有限公司
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