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A method for preparing microbial oil from agricultural waste sweet potato vines

A technology for microbial oil and sweet potato vines, which is applied in the field of microorganisms utilizing agricultural waste sweet potato vines to ferment and produce microbial oil, and achieves the effects of easy collection and processing, simple process and mild preparation conditions

Inactive Publication Date: 2011-12-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But we also found that these nutritional and medicinal values ​​belong to the fresh, young sweet potato stems and leaves, and those sweet potato vines that survived the sweet potato tuber harvest are still largely discarded

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] The preparation method of the microbial oil of the present invention uses sweet potato vines (the general name of sweet potato stems, leaves and vines) discarded at the maturity stage as raw materials, after being crushed or shredded, mixed with a citric acid buffer solution containing cellulase to obtain a solid-liquid mixture, After the solid-liquid mixture is treated at 50°C for 4 days, the hydrolyzate can be obtained by solid-liquid separation, the seed liquid of oleaginous microorganisms is inoculated into the hydrolyzate or the mixture of hydrolyzate and sugar, and the fermentation product is obtained by conventional submerged fermentation. The oily bacteria are collected from the fermentation product, and the microbial oil is obtained by conventional organic solvent extraction method.

[0019] The present invention uses agricultural waste sweet potato vines as the method for raw material preparation microbial grease, comprises the following steps:

[0020] 1. Pre...

Embodiment 1

[0030] Example 1: Preparation of sweet potato vine hydrolyzate (1)

[0031] The air-dried or sun-dried sweet potato vine sample is crushed to about 20 meshes. In a 500ml Erlenmeyer flask, add 25g of dry sample powder and 250ml of citric acid buffer (0.05M, pH4.8), mix well, seal with tin tissue gauze and newspaper, and sterilize at 115°C for 20 minutes. After the mixed sample was cooled, 175ul of cellulase and 250ul of pectinase were added. Add the enzyme mixed solution at 50 degrees, 100rpm vibration treatment for 4 days, then remove the slag with gauze, and further filter the slag removal solution to obtain a clarified sweet potato vine hydrolyzate.

Embodiment 2

[0032] Example 2: Preparation of sweet potato vine hydrolyzate (2)

[0033] Shred fresh sweet potato vines, take 50g shredded sample and add 250ml citric acid buffer (0.05M, pH4.8), homogenize with a juicer to obtain a solid-liquid mixture, transfer the solid-liquid mixture to a 500ml Erlenmeyer flask , sealed with tin tissue gauze and newspaper, and sterilized at 115 degrees for 20 minutes. After the mixed sample was cooled, 175ul of cellulase and 250ul of pectinase were added. Add the enzyme mixed solution at 50 degrees, 100rpm vibration treatment for 4 days, then remove the slag with gauze, and further filter the slag removal solution to obtain a clarified sweet potato vine hydrolyzate.

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PUM

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Abstract

The invention provides a method for preparing microorganism grease by taking agricultural abandoned sweet potato vine as raw material, comprising the following steps of: smashing or shredding mature abandoned sweet potato vine; mixing with citric acid buffer solution containing cellulose to obtain solid-liquid mixture; processing the solid-liquid mixture for 50DEG C for four days; obtaining hydrolyzate after solid-liquid separation; inoculating seeds of microorganism capable of generating oil into the hydrolyzate or mixture of the hydrolyzate and sugar; and obtaining a fermentation product by conventional liquid submerged fermentation. Grease thallus in the fermentation product is collected, and the microorganism grease is obtained with a conventional organic solvent extraction method. With the method, the mature abandoned sweet potato vine is fully utilized, and the method is a resource high-value technology. The sweet potato vine hydrolyzate is prepared with a composite hydrolysis method of cellulose and pectinase, a processing method is moderate, the obtained hydrolyzate has abundant nutrition and high grease conversion rate.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a method for microorganisms to produce microbial oil by fermenting agricultural waste sweet potato vines. Background technique [0002] Oil is not only an important nutrient for sustaining life, but also an important raw material for industrial production. Traditional edible oils and fats are mainly derived from animals or oil plants. With the continuous development of industrial production and the increasing world population, oils and fats are in short supply. In response to this problem, scientists have resorted to bioengineering technology to produce microbial oils through biosynthesis in microorganisms, which has created a new way to open up new oil resources. Microbial oils, also known as single cell oils (SCOs), can be produced by some oil-producing microorganisms such as yeast, fungi, bacteria, microalgae, etc., which are similar to vegetable oils in compos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 詹局妹林辉周启发赵宇华
Owner ZHEJIANG UNIV