Method for rapidly propagating roots of subprostrate sophora by tissue culture

A root tissue and fast technology, applied in the field of plant reproduction, can solve the problems of serious root diseases and insect pests, difficult to achieve large-scale cultivation, low seed yield, etc. Effect

Inactive Publication Date: 2012-01-04
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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Problems solved by technology

Under natural conditions, broad bean roots mainly rely on seeds for reproduction, but during the flowering and pod setting stage, broad bean roots are severely damaged by diseases and

Method used

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  • Method for rapidly propagating roots of subprostrate sophora by tissue culture
  • Method for rapidly propagating roots of subprostrate sophora by tissue culture
  • Method for rapidly propagating roots of subprostrate sophora by tissue culture

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Embodiment Construction

[0018] The technical solutions of the present invention will be further described below in conjunction with embodiments.

[0019] A rapid propagation method of broad bean root tissue culture includes the following steps:

[0020] (1) Selection and disinfection of explants: Take the plump root planting as explants, soak them in a detergent solution for 5 minutes, rinse with tap water for 15-30 minutes, disinfect with 0.1% mercury for 8-10 minutes, sterile water Rinse 3-5 times, and finally remove the surface moisture with sterile filter paper; add 2-3 drops of Tween-20 to every liter of 0.1% mercury;

[0021] (2) Seed germination to obtain sterile test tube plantlets: inoculate the explants obtained in step (1) into MS medium and culture for 30 days, and obtain sterile test tube plantlets after germination; culture temperature 23-27℃, light intensity 1500lux, the light time is 12-14 hours / day; the MS medium contains 0.5mg / L 6-BA, 30g / L sucrose and 3.4g / L agar, and the pH value of the...

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Abstract

The invention relates to a method for rapidly propagating roots of subprostrate sophora by tissue culture. The method provided by the invention comprises the following steps of: (1) taking seeds of the roots of subprostrate sophora as explants to be disinfected; (2) putting the disinfected explants into an MS (Murashige and Skoog) basic culture medium to be induced and germinated to obtain sterile test-tube seedlings; (3) putting the sterile test-tube seedlings into an MS propagating culture medium to carry out a rapid propagating culture on the sterile test-tube seedlings to obtain cluster buds; (4) putting the cluster buds into an MS sound seedling culture medium to carry out a sound seedling culture to obtain strong plants; (5) putting the strong plants into an MS rooting culture medium to be cultured to obtain complete seedlings with roots; and (6) taking the complete seedlings with roots to adopt seedling adaptation and then transplanting the seedlings to a sand bed to grow for one month; and then transplanting the seedlings to a land for growing field crops. The germchit obtained by the method is strong, has a high survival rate, and can supply a lot of good-quality germchits of the roots of subprostrate sophora in short order; and the method effectively solves the problem of a large-scale seedling culture of the roots of subprostrate sophora.

Description

Technical field [0001] The invention relates to a plant propagation method, in particular to a rapid propagation method of broad bean root tissue culture. Background technique [0002] Guangdougen, also known as Shandougen and Nandougen, is the dried root and rhizome of the leguminous plant Sophora tonkinensis Gapnep. It is distributed in southern my country, and Guangxi has the largest yield. It has the effects of clearing fire and detoxification, reducing swelling and pain, and can treat carbuncle, throat wind, throat numbness, gum swelling and pain, asthma and fever cough, hepatitis, constipation, jaundice, diarrhea, hemorrhoids, alopecia, scabies and mosquitoes , Dog bites, etc. The pharmaceutical industry uses broad bean root as a raw material to develop a large number of injections for the treatment of hepatitis, tablets for sore throat and anti-tumor Chinese patent medicines. [0003] The geographical distribution range of broad bean root is quite limited, and it often gro...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 缪剑华韦坤华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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