Formula and preparation method of compound coptis injection
A technology of injection and Coptis chinensis, applied in the field of new-type compound Coptis chinensis injection for intramuscular injection of livestock and poultry and its preparation, can solve problems such as imperfect extraction method and preparation process, unstable content of active ingredients and curative effect, poor clarity of compound injection, etc. , achieve good market prospect, better effect and low cost
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Embodiment 1
[0026] Embodiment 1 Below in conjunction with preferred embodiment, the specific implementation mode provided according to the present invention is described in detail as follows:
[0027] A compound Coptis rhizome injection for livestock and poultry, which is prepared by extracting, precipitating, separating and refining 42.86 grams of Coptis chinensis, 14.29 grams of Cortex Phellodendri, 28.57 grams of Gardenia, 14.29 grams of Scutellaria baicalensis and 1500 grams of ultrapure water.
[0028] A preparation method of Compound Coptis Rhizoma Injection for livestock and poultry, carried out according to the following steps:
[0029] (1) Weigh 42.86 grams of Coptidis Rhizome, 14.29 grams of Phellodendron Phellodendri, 28.57 grams of Gardenia Fructus Jasmine, and 14.29 grams of Scutellaria baicalensis, which have been dried, crushed and sieved through a 40-mesh sieve.
[0030] (2) Soak the mixed materials of the four medicinal powders in ultra-pure water with a total weight of 1...
Embodiment 2
[0054] In vitro bacteriostatic test of embodiment 2 Compound Coptidis Injection
[0055] (1) Determination of minimum inhibitory concentration (MIC)
[0056] Methods The test tube dilution method was used. The compound Coptis chinensis injection was diluted to two-fold decreasing concentration, and the drug concentrations in test tubes 1 to 9 were 0.5, 0.25, 0.125, 0.0625, 0.0313, 0.0156, 0.0078, 0.0039, and 0.00195 g / ml. Prepare 5 drug-resistant Staphylococcus aureus strains according to the general test method, add 0.1mL of bacterial liquid to each tube, add the drug liquid to the No. 10 test tube, as a negative control, add the bacterial liquid to the No. 11 test tube No drug solution was used as a positive control. Each tube was cultured with shaking at 35°C for 18 hours, and the minimum inhibitory concentration (MIC) of the drug was observed and determined.
[0057] Results The results of MIC determination of Compound Huanglian Injection are shown in Table 1. The resu...
Embodiment 3
[0066] In vitro drug resistance inhibition test of embodiment 3 Compound Coptidis Injection
[0067] (1) Plate digging test
[0068] Methods Drug-resistant Staphylococcus aureus was cultured in MH liquid medium for 20 hours, and after dilution, 0.1 ml was taken and spread on MH agar medium, and 4 holes were punched with a puncher, and the bottom was sealed. The distance between each hole was about 20 mm. Add 0.05 ml of Compound Coptidis Injection to 2 symmetrical wells, add 0.05 ml ofloxacin or ciprofloxacin (concentration and dosage refer to drug susceptibility test method) into adjacent symmetrical wells, incubate for 20 hours, and observe the results.
[0069] The results showed that the bacteria grew around the two holes, but there was an area where the bacteria did not grow between the two holes, which was regarded as effective inhibition of drug resistance; the bacteria around the two holes and between the holes grew into flakes, which was regarded as the criterion for j...
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