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Preparation method for cynarin

A technique for artichoke and artichoke leaves, which is applied in the field of natural medicine separation, can solve the problems of high cost and high equipment requirements, and achieves the effects of good quality, good impurity removal effect and high content.

Inactive Publication Date: 2012-03-14
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process uses ethanol or acetone for extraction, which requires high equipment and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take the artichoke leaf raw material and crush it, weigh 5kg and put it into a small extraction tank, add 40kg of water to reflux for extraction for 1 hour, extract 3 times, add 100g101 fruit juice clarifier to the combined extract, filter for 12 hours, add the liquid to 4L D301 anion resin column for adsorption , the flow rate is controlled at 8L / h. After the adsorption is completed, wash the neutral with water. Take 24L of 0.5% acetic acid solution to elute the active ingredients. The elution rate is 2L / h. Collect the eluate and add 2LAB-8, macroporous resin Column adsorption, the flow rate is 3L / h. After the adsorption is completed, wash the neutral with water, and then measure 16L of 40% ethanol solution for elution, the elution flow rate is 2L / h, the eluent is concentrated under reduced pressure to a density of 1.3, and the crystal is placed on it, and the crude crystal is filtered out , and then sequentially refluxed with deionized water and 90% ethanol to dissolve...

Embodiment 2

[0027] Take the artichoke leaf raw material and crush it, weigh 5kg and put it into a small extraction tank, add 75kg of water to reflux for extraction for 2 hours, extract twice, add 200g101 fruit juice clarifier to the combined extract, filter for 10 hours after precipitation, add the liquid to 4L D280 anion resin column for adsorption , the flow rate is controlled at 8L / h, after the adsorption is completed, wash neutral with water, take 16L of 1% acetic acid solution to elute the active ingredients, the elution rate is 2L / h, collect the eluate, add 2LAB-8, and adsorb on a macroporous resin column , the flow rate is 3L / h. After the adsorption is completed, wash the neutral with water, and then measure 10L of 70% ethanol solution to elute. The elution flow rate is 2L / h. Then deionized water and 99% ethanol were refluxed to dissolve the crystals successively, and the crystals were dried under vacuum and reduced pressure to obtain 12 g of cynarin with a content of 97%.

Embodiment 3

[0029] Take the artichoke leaf raw material and crush it, weigh 5kg and put it into a small extraction tank, add 40kg of water to reflux for extraction for 1 hour, extract 3 times, add 300ml of ZTC1+1 sedimentation agent to the combined extract, settle for 14 hours and filter, and add the liquid to a 4L D290 anion resin column Adsorption, the flow rate is controlled at 8L / h, after the adsorption is completed, wash the neutral with water, take 20L of 0.8% acetic acid solution to elute the active ingredient, the elution rate is 1L / h, collect the eluate, add 2LAB-8, macroporous Resin column adsorption, the flow rate is 4L / h. After the adsorption is completed, wash the neutral with water, and then measure 14L of 50% ethanol solution for elution, the elution flow rate is 2L / h, and the eluent is concentrated under reduced pressure to a density of 1.3. The crude crystals were refluxed with deionized water and 95% ethanol to dissolve the crystals, and the crystals were dried under vacu...

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PUM

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Abstract

The invention relates to a preparation method for cynarin. The preparation method includes the following process steps: artichoke leaves as material are ground, aqueous solution is added for reflux extraction, precipitator is added to extract for clarification, alkalescent anion resin is added for adsorption, 0.5 to 1 percent of acetic acid solution is used for elution, eluent is then added into macroporous resin for adsorption, 40 to 70 percent of alcohol solution is used for elution, eluent is concentrated and crystallized, macrocrystalline is then recrystallized by deionized water and alcohol, and after vacuumization pressure reduction and drying, the product is prepared. The process flow of the preparation method is easy to operate, the energy consumption of the production process is low, pollution is little, the cost of the prepared product is low, the quality of the product is good, and drugs or health-care products can be prepared according to the invention.

Description

Technical field: [0001] The invention belongs to the technical field of separation of natural medicines, in particular to a method for preparing cynarin by using ion resin and macroporous resin. Background technique: [0002] Cynarin is also called artichoke acid, artichoke, phenolic acid substances. [0003] Molecular formula: C 25 h 24 o 12 [0004] Molecular weight: 516.45 [0005] Physical and chemical properties: crystal (dilute acetic acid), mp.225-227°C. Optical rotation [α] -59 (c=2, methanol). Slightly soluble in cold water, easily soluble in boiling water, soluble in glacial acetic acid and alcohols. [0006] Cynarin can promote the regeneration of liver cells, help the secretion of bile in the body, participate in the metabolism of cholesterol and fat, and has the functions of promoting gallbladder, protecting liver and lowering cholesterol. [0007] Existing methods for extracting cynarin are few, and most of them are crude extracts, especially the method...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/56C07C67/52
Inventor 李法庆杨成东刘东锋
Owner 苏州宝泽堂医药科技有限公司
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