Method for rapid propagation of Jatropha curcas
A kind of Tongzi, fast technology, applied in the field of rapid propagation of Jatropha japonica, can solve problems such as unpublished, unable to achieve industrial production, etc., and achieve the effect of multiplying multiples
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Embodiment 1
[0049] In this example, wild jatropha seeds in Yuanmou, Yunnan were used as materials. Please refer to Table 1 for the total number of samples and the number of survivors after each treatment step. The main process steps are as follows:
[0050] Proliferation induction step S2: Cut off the differentiated regenerated shoots and insert them into the proliferation medium MP8, namely MS+1.0 mg / L 6-benzylpurine+0.01 mg / L indolebutyric acid+0.025 mg / L silver nitrate , and added 3% sucrose and 0.7% agar, pH 5.8, the temperature was controlled at 24°C, and the light time was 12 h / d for 30 days, and the light intensity was 1800 lx.
[0051] Elongation culture step S3: Excise the proliferated cluster buds and inoculate them into elongation medium SEB3, namely MS+0.3 mg / L 6-benzylpurine and 0.25 mg / L gibberellin, and add 3% sucrose and 0.7% agar, pH 5.8, to induce elongation of stems and leaves. The culture temperature was controlled at 24-26°C, the light time was 12 h / d and cultured fo...
Embodiment 2
[0055] In this example, wild jatropha seeds in Yuanmou, Yunnan were used as materials. Please refer to Table 1 for the total number of samples and the number of survivors after each treatment step. The main process steps are as follows:
[0056] Proliferation induction step: cut the differentiated regenerated shoots and insert them into the proliferation medium MP16, that is, MS+2.0 mg / L 6-benzylpurine+0.1 mg / L indolebutyric acid+1 mg / L silver nitrate, Add 3% sucrose and 0.7% agar, pH 5.8, culture temperature is controlled at 26°C, light time is 12 h / d and culture is 35 days, light intensity is 2000 lx.
[0057] Elongation culture step: excise the proliferated cluster buds and inoculate them into elongation medium SEB10, namely MS +2 mg / L 6-benzylpurine and 1 mg / L gibberellin, and add 3% sucrose and 0.7% agar, pH 5.8, induced stem and leaf elongation, the culture temperature was controlled at 26°C, the light time was 12 h / d and the light intensity was 1800 lx for 20 days.
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Embodiment 3
[0061] In this example, wild jatropha seeds in Yuanmou, Yunnan were used as materials. Please refer to Table 1 for the total number of samples and the number of survivors after each treatment step. The main process steps are as follows:
[0062] Proliferation induction step: cut the differentiated regenerated shoots and insert them into the proliferation medium MP0, that is, MS+0.1 mg / L 6-benzylpurine+0.01 mg / L indolebutyric acid+5 mg / L silver nitrate, Add 3% sucrose and 0.7% agar, pH 5.8, culture temperature is controlled at 26°C, light time is 12 h / d and culture is 40 days, light intensity is 2000 lx.
[0063] Elongation culture step: excise the proliferated cluster buds and inoculate them into elongation medium SEB1, namely MS +0.1 mg / L 6-benzylpurine and 0.5 mg / L gibberellin, and add 3% sucrose and 0.7% agar, pH 5.8, induced stem and leaf elongation, the culture temperature was controlled at 24-26 °C, the light time was 12 h / d and the light intensity was 2000 lx for 30 da...
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