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Biocontrol coniothyrium minitans Chy-1C-1 suspending agent for preventing and treating sclerotinia rot, and its preparation and application

A technology of chy-1c-1 and Conchicoides, applied in biochemical equipment and methods, microorganisms, fungi, etc., can solve problems such as unreported production technology, achieve environmental friendliness, improve dispersing ability, and anti-ultraviolet ability. strong effect

Inactive Publication Date: 2013-09-25
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production technology of my country's shell mold preparation has not been reported.

Method used

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  • Biocontrol coniothyrium minitans Chy-1C-1 suspending agent for preventing and treating sclerotinia rot, and its preparation and application
  • Biocontrol coniothyrium minitans Chy-1C-1 suspending agent for preventing and treating sclerotinia rot, and its preparation and application
  • Biocontrol coniothyrium minitans Chy-1C-1 suspending agent for preventing and treating sclerotinia rot, and its preparation and application

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1: Isolation and identification of Shell mold Chy-1C-1 bacterial strain

[0035] In May 1995, the applicant isolated and screened from the soil of the vegetable plantation in Changyang County, Hubei Province, China, and obtained a strain of the shell mold that has a strong parasitic and rot-causing effect on the sclerotia sclerotia. The applicant named it shield shell Mold (Coniothyrium minitans) Chy-1C-1. Shell mold Chy-1C-1 of the present invention separates and screens and adopts sclerotinia sclerotinia sclerotium trapping method (Adames etc., 1980) and the dilution plate method, strain identification work is with reference to Wei Jingchao " fungus identification handbook " Shanghai Science and Technology Publishing House, The method introduced in the 1979 edition.

[0036] The bacterial strain was submitted to the China Center for Type Culture Collection (CCTCC) located in Wuhan University, Wuhan City, Hubei Province for preservation on September 17, 2010,...

Embodiment 2

[0037] Embodiment 2: the solid fermentation of shell mold Chy-1C-1

[0038] Produce according to the solid fermentation method of routine report, wherein solid fermented material formula (by mass percentage) is wheat bran 42.31%, corn flour 36.53%, rice husk 21.16%, replenishing moisture to moisture content is 85.12%, above-mentioned fermented material is in Sterilize under high pressure steam at 121°C for 2 hours, inoculate 1×10 immediately after sterilization 6 ml -1 Concentration of C. shieldiae Chy-1C-1 spore liquid, stir evenly, it is carried out shallow plate fermentation, and culture room temperature is 20 ℃, fermented and cultivated for 12 days, obtains C. shieldiae Chy-1C-1 spore of the present invention (1 ×10 10 g -1 )mixture.

Embodiment 3

[0039] Embodiment 3: the preparation of shell mold Chy-1C-1 suspension formulation

[0040] The spore mixture of C. shieldum Chy-1C-1 obtained in Example 2 is washed with water, filtered to remove solid impurities, and the spore liquid is centrifugally concentrated, and then the spore liquid obtained by solid fermentation of C. shieldiae Chy-1C-1 is adjusted to the concentration 2×10 9 ml -1 . The percentages described in the C. shield Chy-1C-1 suspension concentrate in the present embodiment are all mass percentages. Add sodium lignosulfonate or calcium lignosulfonate 3%, xanthan gum 0.3%, streptomycin 0.1%, ethylene glycol 0%, dimethyl in the described shell mold Chy-1C-1 spore liquid Base silicone oil 1%, ascorbic acid (vitamin C) 0.05%, obtain the described suspending agent after stirring and mixing uniformly (note that the above-mentioned auxiliary agent should be added slowly and constantly stirring when the suspending agent is prepared).

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Abstract

The invention specifically relates to separation and screening of a biocontrol coniothyrium minitans strain for preventing and treating sclerotinia rot, a suspending agent prepared from the strain and application of the same, belonging to the technical field of microbial pesticides. The strain of coniothyrium minitans Chy-1C-1 is preserved in China Center for Type Culture Collection and is assigned the accession number of CCTCC No. M2010238. The suspending agent is prepared by mixing bacteria-containing spore liquid produced through solid fermentation of the strain of coniothyrium minitans Chy-1C-1 with a wetting dispersant, a viscosity conditioning agent, an antiseptic, an anti-freezing agent and an anti-foaming agent. The invention also provides a preparation method for the suspending agent and application of the suspending agent of coniothyrium minitans Chy-1C-1 in preventing and treating sclerotinia rot of rape. The strain of coniothyrium minitans Chy-1C-1 and the suspending agent thereof in the invention have a good biocontrol effect on sclerotinia rot of rape.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and specifically relates to the breeding of a biocontrol fungus Chy-1C-1 strain for preventing and treating sclerotinia, a microbial preparation containing the strain, that is, a suspension, its preparation method and application. Background technique [0002] Sclerotinia sclerotiorum caused by Sclerotinia sclerotiorum (Lib.) de Bary] is an important disease worldwide (Purdy, 1979). This pathogen poses a great threat to the yield and quality of some important economic crops such as rapeseed, soybean, sunflower, lettuce and so on. Sclerotinia sclerotiorum caused by Sclerotinia sclerotiorum is also an important disease of rapeseed in my country, especially in the Yangtze River Basin and the southeast coastal rapeseed producing areas. The incidence rate in general years is 10%-30%, and it can reach more than 80% in severe years. After the rape is infected, the yield is reduced by 11%-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 李国庆姜道宏唐善军杨龙
Owner HUAZHONG AGRI UNIV
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