Biocontrol coniothyrium minitans Chy-1C-1 suspending agent for preventing and treating sclerotinia rot, and its preparation and application

A technology of chy-1c-1 and shell mold, which is applied in the direction of application, biocide, fungicide, etc., can solve the problems of unreported production technology, achieve environmental friendliness, improve suspension stability, and stabilize product quality.

Inactive Publication Date: 2012-04-11
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production technology of my country's shell mold preparation has not been reported.

Method used

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  • Biocontrol coniothyrium minitans Chy-1C-1 suspending agent for preventing and treating sclerotinia rot, and its preparation and application
  • Biocontrol coniothyrium minitans Chy-1C-1 suspending agent for preventing and treating sclerotinia rot, and its preparation and application
  • Biocontrol coniothyrium minitans Chy-1C-1 suspending agent for preventing and treating sclerotinia rot, and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Isolation and identification of Chy-1C-1 strain

[0035] The applicant isolated and screened from the soil of vegetable plantations in Changyang County, Hubei Province, China in May 1995, and obtained a Conotodium scutellaria strain that has strong parasitic and rot-causing effects on the sclerotia of Sclerotinia sclerotiorum. The applicant named it as sclerotia sclerotiorum Coniothyrium minitans Chy-1C-1. The isolation and screening of Chy-1C-1 of Conidon scutellaria in the present invention adopts sclerotinia sclerotiorium sclerotia trap method (Adames et al., 1980) and dilution plate method. For strain identification, please refer to Wei Jingchao "Fungi Identification Manual" Shanghai Science and Technology Press, The method introduced in the 1979 edition.

[0036] The strain was submitted to the China Center for Type Culture Collection (CCTCC) in Wuhan University, Wuhan City, Hubei Province for the patent procedure on September 17, 2010, and its deposit numbe...

Embodiment 2

[0037] Example 2: Solid fermentation of Conchorium scutellare Chy-1C-1

[0038] According to the conventionally reported solid fermentation method, the solid fermentation material formula (by mass percentage) is 42.31% wheat bran, 36.53% corn flour, 21.16% rice husk, supplemented with water to a moisture content of 85.12%, and the above fermentation material is Autoclave sterilization at 121℃ for 2h, immediately inoculate 1×10 after sterilization 6 ml -1 Concentrated Chy-1C-1 spore solution of Conchochon scutellariae Chy-1C-1, stir it evenly, and ferment it in a shallow tray. The temperature of the culture room is 20℃, and the fermentation culture is 12 days to obtain the Chy-1C-1 spores (1 ×10 10 g -1 )mixture.

Embodiment 3

[0039] Example 3: Preparation of Suspension Containment Formula of Conchorium scutellaria Chy-1C-1

[0040] The spore mixture of C. scutellae Chy-1C-1 obtained in Example 2 was washed with water, filtered to remove solid impurities, and the spore liquid obtained by centrifugation was concentrated, and then the spore liquid obtained by solid fermentation of C. scutellar Chy-1C-1 was adjusted to a concentration 2×10 9 ml -1 . The percentages of the Chy-1C-1 Suspension Concentrate of Conchorium scutellaria in this embodiment are all mass percentages. Add 3% sodium lignosulfonate or calcium lignosulfonate, 0.3% xanthan gum, 0.1% streptomycin, 0% ethylene glycol, and dimethyl sulfonate to the spore solution of Chy-1C-1. Base silicone oil 1%, ascorbic acid (vitamin C) 0.05%, stir and mix uniformly to obtain the suspending agent (note that the suspending agent should be slowly and continuously stirred to add the above additives).

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Abstract

The invention specifically relates to separation and screening of a biocontrol coniothyrium minitans strain for preventing and treating sclerotinia rot, a suspending agent prepared from the strain and application of the same, belonging to the technical field of microbial pesticides. The strain of coniothyrium minitans Chy-1C-1 is preserved in China Center for Type Culture Collection and is assigned the accession number of CCTCC No. M2010238. The suspending agent is prepared by mixing bacteria-containing spore liquid produced through solid fermentation of the strain of coniothyrium minitans Chy-1C-1 with a wetting dispersant, a viscosity conditioning agent, an antiseptic, an anti-freezing agent and an anti-foaming agent. The invention also provides a preparation method for the suspending agent and application of the suspending agent of coniothyrium minitans Chy-1C-1 in preventing and treating sclerotinia rot of rape. The strain of coniothyrium minitans Chy-1C-1 and the suspending agentthereof in the invention have a good biocontrol effect on sclerotinia rot of rape.

Description

Technical field [0001] The present invention belongs to the technical field of microbial pesticides, and specifically relates to the selection and breeding of a biocontrol bacteria Chy-1C-1 strain of Conidia sclerotiorum for preventing and treating sclerotinia, a microbial preparation containing the strain, that is, a suspension, and a preparation method and application thereof. Background technique [0002] Rapeseed sclerotinia disease caused by Sclerotinia sclerotiorum (Lib.) de Bary is an important disease with worldwide distribution (Purdy, 1979). This pathogen poses a great threat to the yield and quality of some important economic crops such as rapeseed, soybean, sunflower, lettuce and other crops. Sclerotinia sclerotiorum caused by sclerotinia sclerotiorum is also an important disease on rapeseed in my country, especially in the Yangtze River Basin and the southeast coastal rapeseed producing areas. The incidence rate is 10%-30% in general years, and can reach more than 8...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01N25/04A01P3/00C12R1/645
Inventor 李国庆姜道宏唐善军杨龙
Owner HUAZHONG AGRI UNIV
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