Ziziphus jujube growth-promoting rhizobacteria Bacillus subtilis and its uses
A Bacillus subtilis and rhizosphere growth-promoting technology, applied in the direction of application, bacteria, microorganisms, etc., can solve the problems of screening and application of winter jujube rhizosphere growth-promoting bacterial strains that have not been reported, so as to achieve excellent strain resources and improve ecology Environment, growth-promoting effect
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Embodiment 1
[0017] Example 1 Screening of Dongzao rhizosphere growth-promoting Bacillus subtilis
[0018] (1) Screening process:
[0019] By gradient dilution method, 87 isolates were isolated from rhizosphere soil samples of Dongzao demonstration garden of Binzhou Forestry Bureau, Shandong Province. Purify the isolated bacteria by the three-section line method, and judge whether the strain is purified by microscopic examination, number the purified bacteria, pick a single colony, and transfer it to an LB slant for future use. 23 bacterial strains with better green-preserving effect were screened out by the green-preserving method, combined with the radish cotyledon weight-increasing method, and finally one bacterial strain with good growth-promoting effect was screened out, which was designated as DZ1.
[0020] (2) Colony characteristics and cell morphology:
[0021] After cultured on LB plates for 24 hours, DZ1 was round and opaque with a smooth surface. The cell size of the bacteria...
Embodiment 2
[0026] Embodiment 2 pot experiment
[0027] Activate the preserved winter jujube rhizosphere-promoting Bacillus subtilis DZ1 on the LB slant, pick a ring and insert it into the beef extract peptone medium, shake and cultivate it at 37°C and 180rpm for 2 days, and the concentration of the bacterium adjustment solution is 2.5× 10 8 cfu / ml (suspension A), for later use. Soak the winter jujube seedlings in the suspension A and then plant them. Adjust the concentration of the prepared suspension A to 1×10 8 cfu / ml (suspension B), take 50ml of suspension B and evenly water around the roots of winter jujube seedlings to inoculate annual winter jujube seedlings. After dipping the roots of winter jujube seedlings in sterile water, water the same amount of sterile water as a control. Under normal management, the fresh weight, capillary root weight, seedling height and ground diameter of the root system were measured after the jujube seedlings stopped growing that year. The results ...
Embodiment 3
[0031] Embodiment 3 field test
[0032] Dongzao rhizosphere growth-promoting Bacillus subtilis DZ1 was shaken and cultured in a reciprocating shaker at 37°C and 180rpm for 2 days, and the concentration of the bacteria adjustment solution was 5.0×10 8 cfu / ml, inoculated into decomposed chicken manure to make winter jujube bio-organic fertilizer (0.5×10 8 cfu / g). Take the same amount of decomposed chicken manure as a control, and apply 8.5kg to each tree. The method of fertilization is furrow fertilization on the southeast, northwest and four sides. The length, width and height of the ditch are 60, 30 and 20 cm respectively. The bio-organic fertilizer of winter jujube is fully mixed with the excavated soil and then backfilled. Every time 30 trees were treated, a protection line was set. After two years of application, the total amount of amino acids in the rhizosphere soil of Dongzao was determined by formaldehyde titration, the total sugar content was determined by anthrone ...
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