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Relevant protein TaOPR for regulating and controlling cracking of plant anther as well as gene and application thereof

A related protein and plant flower technology, which is applied to the regulation of plant anther dehiscence-related protein TaOPR and its gene and application fields, and can solve the problems that fertilization cannot be completed in time, pollen cannot be dispersed in time, and anther dehiscence is delayed.

Active Publication Date: 2012-05-02
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sanders PM et al. screened a male sterile Arabidopsis mutant strain through T-DNA insertion technology in 2000. The anther dehiscence of the mutant strain was delayed so that pollen could not be shed in time, and fertilization could not be completed in time.
However, there is no report on OPR in wheat

Method used

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  • Relevant protein TaOPR for regulating and controlling cracking of plant anther as well as gene and application thereof
  • Relevant protein TaOPR for regulating and controlling cracking of plant anther as well as gene and application thereof
  • Relevant protein TaOPR for regulating and controlling cracking of plant anther as well as gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1: cDNA cloning and semi-quantitative analysis of anther dehiscence-related gene TaOPR in the wheat jasmonic acid metabolic pathway

[0095] The anthers of the hybrid wheat variety Jingmai No. 7 (Jingshenmai 2009003) were taken, and the total RNA of the anthers was extracted by Trizol method. 5'RACE kit (5'RACE System for Rapid Amplification of cDNA Ends Kit) (GIBCOBRL, CAT.NO.18374-058) and 3'RACE kit (3'RACE System for Rapid Amplification of cDNA Ends Kit) ( GIBCOBRL, CAT.NO.18373-019) obtained the TaOPR gene, the full length of which is 845kb.

[0096] The total RNA of the anthers of hybrid wheat variety Jingmai No. 7 (Jingshenmai 2009003) was extracted by Trizol method, and cDNA was obtained by reverse transcription with superscript II (purchased from Invitrogen Company) reverse transcriptase. Primers P1 and P2 were designed according to the sequence of TaOPR gene coding region. Using the cDNA obtained by reverse transcription as a template, PCR amplificat...

Embodiment 2

[0102] Embodiment 2: Construction of TaOPR monocot transgene expression vector

[0103] 1. Construction of recombinant expression vector

[0104] Construction of pAHC-TaOPR monocotyledon recombinant expression vector: Using the cDNA obtained by reverse transcription of the total RNA of hybrid wheat variety Jingmai No. 7 (Jingshenmai 2009003) as a template, PCR was carried out with specific primers containing BamHI and SacI linker sequences Amplify; then BamHI and SacI double-digest the PCR product, recover, and insert the digested product between the BamHI and SacI restriction sites behind the CaMV 29A promoter of the vector pAHC25 in the forward direction to obtain the recombinant vector pAHC-TaOPR.

[0105] The primer sequences are as follows:

[0106] OPR[BamHI]: 5′-CGGGATCCACGAGGGCAGTGC-3′

[0107] OPR[SacI]: 5′-CGAGCTCTACGACTCGTCCTC-3′

[0108] 2. Acquisition and Identification of Transgenic Wheat

[0109] 1) The recombinant plasmid pAHC-TaOPR obtained above was media...

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Abstract

The invention relates to the field of genetic engineering, in particular to a relevant protein TaOPR for regulating and controlling the cracking of a plant anther as well as a gene and application thereof. The invention provides the relevant protein TaOPR for regulating and controlling the cracking of the plant anther. The amino acid sequence of the relevant protein TaOPR is shown as SEQ ID NO.1.In addition, the invention also provides the gene for coding the relevant protein for regulating and controlling the cracking of the plant anther. The amino acid sequence of the gene is shown as SEQ ID NO.2 or SEQ ID NO.3. Furthermore, the invention provides a recombinant vector containing the gene and application of the relevant protein TaOPR. The gene provided by the invention is correlated with the fertility of the plants, can be used for improving the cracking rate and the setting percentage of the plant anther and has very important theoretical and actual significances for increasing theyield of grains and developing the two-line hybrid wheat theory.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular, the invention relates to a protein TaOPR related to regulating plant anther dehiscence and its gene and application. Background technique [0002] In modern agricultural production, people widely use heterosis to improve varieties. Heterosis refers to the phenomenon that the first generation of hybrids produced by the hybridization of two parents with different genetic compositions has advantages over their parents in vigor, growth potential, fecundity, stress resistance, yield and quality. Utilization of heterosis is considered to be the main way to greatly increase the wheat production level in the future. Male sterility is a ubiquitous phenomenon in the plant world. Male sterility refers to the sterility of plants caused by the inability to produce functional anthers, pollen or male gametes. [0003] Jasmonates (JA), as an important plant hormone for plants to respond to ex...

Claims

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Application Information

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IPC IPC(8): C12N9/02C12N15/53C12N15/63A01H5/00
Inventor 张立平向群刘泽涛马骅赵昌平唐忠辉苑少华高世庆
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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