Sample analysis method and assay kit for use in the method

A detection kit and analysis method technology, applied in the field of nucleic acid analysis, can solve problems such as large number of samples and increase in chip time detection cost, and achieve the effects of rapid analysis, reduced inspection cost, and simple analysis

Inactive Publication Date: 2014-04-30
KK TOSHIBA
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Problems solved by technology

[0003] On the other hand, for example, in the field of infectious diseases, etc., sometimes the number of genes studied is small, but the number of samples is large
However, in the prior art, it is necessary to use a plurality of chips according to the number of samples, so the detection cost including chips, labor, and time increases

Method used

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  • Sample analysis method and assay kit for use in the method
  • Sample analysis method and assay kit for use in the method
  • Sample analysis method and assay kit for use in the method

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Embodiment approach

[0045] Embodiments of the present invention will be described below.

[0046] figure 1 is a diagram showing tag sequence introduction primers and nucleic acid probes.

[0047]

[0048] Such as figure 1 A sequence that binds to the primer-binding site of the template nucleic acid and a tag sequence for analyzing multiple samples are introduced into the primer as exemplified by the tag-introducing F primer.

[0049] A different sequence is used for the tag sequence depending on the sample. For example, when a tag sequence of 5 bases is prepared and the tag sequence is composed of 4 types of bases, A, T, C, and G, there are 45, that is, 1024 types of tag sequences as candidates. However, since there is a high risk of mismatch hybridization when using tag sequences different in one base, it is preferable to prepare tag sequences different in sequence by two or more bases. As for the number of label sequences to be prepared, it is only necessary to prepare the number of sampl...

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Abstract

One embodiment is related to a method of analyzing plural samples. The method includes amplifying a plurality of samples using a first primer and second primer, wherein the first primer includes a tag sequence having a sequence different from a sample to one another and wherein a second primer used in pair with the first primer in independent reaction systems for the respective samples to obtain an amplified product in which the tag sequence is introduced, mixing amplified products obtained in the plurality of reaction systems, making the mixed amplified product react with a nucleic acid probe immobilized on a substrate, and detecting the amount of hybridization that has occurred.

Description

technical field [0001] The present invention relates to methods for analyzing nucleic acids. Background technique [0002] DNA chips are immobilized on glass slides and / or silicon substrates of several cm each with 10-10 5 A device in which a DNA nucleic acid strand is used as a probe. When analyzing a sample using a DNA chip, first, the contained nucleic acid is labeled with a fluorescent dye, a radioactive isotope, or the like, and then reacted with a probe on the chip. Hybridization occurs if the nucleic acids in the sample contain nucleic acids that are complementary to the probes on the chip. The sequences and immobilized positions of the probes immobilized on the chip are well defined. Therefore, the nucleic acid sequence contained in a sample can be identified by specifying the position on the chip where a signal derived from a marker is obtained (Non-Patent Document 1). A DNA chip is a very useful device (detection device) for analyzing a plurality of genes in on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/09
CPCC12Q1/6853C12Q1/6809C12Q1/6837C12Q2525/161C12Q2525/301C12Q2537/143C12Q2563/179C12Q2565/514
Inventor 中村奈绪子桥本幸二源间信弘
Owner KK TOSHIBA
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