Application of IgAN glomerular sclerosis rat model

A technology of glomerulosclerosis and rat model, applied in medical preparations containing active ingredients, preparations for in vivo experiments, animal husbandry, etc. Genetic engineering models and spontaneous models are expensive and expensive, so as to delay the time of kidney disease, reduce huge medical expenses, and shorten the experimental cycle

Inactive Publication Date: 2012-06-20
SHANGHAI PUTUO DISTRICT CENT HOSPITAL
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Problems solved by technology

[0020] Due to the differences between experimental animals and humans, and the etiology of IgA nephropathy has not yet been definitively answered, so many experimental animal models of IgA nephropathy currently exist are difficult to be completely satisfactory
For example, secondary and mixed models are mixed with many other factors, which cannot simulate the primary process of IgA nephropathy well; genetically engineered models and spontaneous models are too expensive and difficult to promote in China, etc.
At present, only SEB (or SEB-based)-induced models are close to the pathogenesis of human IgA nephropathy, reflecting some of its pathological changes, but the disadvantage of this model is that the formation of glomerular fibrosis takes a long time

Method used

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  • Application of IgAN glomerular sclerosis rat model

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Experimental program
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Effect test

Embodiment 1

[0031] 1. Co-infection.

[0032] After the SD male rats were adaptively fed for 1 week, they began to give 40mg / ml BSA acidified aqueous solution for gavage every other day, and in the 2nd to 4th weeks, injected SEB 0.08mg / ml. Adjuvant 0.2ml / only.qw.

[0033] 2. 5 / 6 nephrectomy.

[0034]On the fifth week, 2 / 3 of the left nephrectomy was performed. After anesthesia, the patient was fixed on the operating table in a prone position. The surgical area on the back was routinely disinfected, and a drape was spread. A straight incision on the left side of the back was used to incise the skin, and the psoas major muscle was separated to enter the retroperitoneum. , find the kidney, and use hemostatic forceps to separate the fat capsule and renal pedicle around the kidney, cut the subcutaneous tissue and capsule layer by layer, separate the adrenal gland with special care to avoid damage, fully expose the left kidney, expose the renal pedicle, and use fiber hemostatic forceps Clamp t...

Embodiment 2

[0038] 1. Establishment of IgAN glomerulosclerosis rat model.

[0039] After 96 SD male rats were fed adaptively for 1 week, they were divided into 4 groups according to the random number table according to their body weight, namely: sham operation group (group A), 5 / 6 nephrectomy group (group B), and combined infection group (group B). Group C), combined infection combined with 5 / 6 nephrectomy group (group D), 24 rats in each group.

[0040] (1). Group A: sham operation group. The same amount of normal saline was given during intragastric administration and intravenous injection, and a sham operation was performed, that is, after intraperitoneal injection of anesthesia in rats, bilateral kidneys were freed, perirenal fat was stripped, and the kidneys were removed. Reset, all the other operations are the same as the 5 / 6 nephrectomy method in the first embodiment.

[0041] (2). Group B: 5 / 6 nephrectomy group. The same amount of normal saline was given during intragastric admin...

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Abstract

The invention discloses an application of an IgAN glomerular sclerosis rat model. The establishing method of the IgAN glomerular sclerosis rat model comprises the following steps of: step 1, selecting a rat, lavaging the rat with bovine serum albumin acidulated aqueous solution on the next day after the rat is raised for 1 week, injecting staphylococcus aureus B-type enterotoxin solution from the vein of the penis of the rat, and injecting complete a Freund's adjuvant from the enterocoelia of the rat during the period from the second week to the fourth week; and step 2, nephrectomizing the rat for two times at the fifth week and the sixth week, and cutting five sixths of the whole kidney at two times. The application of the rat model comprises the application in pathology research of an IgAN glomerular sclerosis process. According to the IgAN glomerular sclerosis rat model disclosed by the invention, the manufacturing time for the IgAN glomerular sclerosis rat model is shortened, the generation and the development of nephridial tissue fibrillation are enhanced, and the scientific research work of IgA nephropathy medical science can be better served.

Description

technical field [0001] The present invention relates to the use of a glomerulosclerosis model, in particular to the use of a rat model of IgAN glomerulosclerosis. Background technique [0002] IgA nephropathy is glomerulonephritis characterized by IgA deposition in the mesangial region of the glomerulus, some scholars classify it as a systemic disease, but most scholars divide IgA nephropathy into primary and secondary Episodic IgA nephropathy is purpura nephritis, lupus nephritis and hepatitis B / cirrhosis-associated nephritis. IgA nephropathy is the most common primary glomerular disease worldwide. Even in the United States, where the incidence of IgA nephropathy is relatively low, IgA nephropathy still accounts for the first among primary glomerular diseases among adults aged 20-39 bit. Because IgA nephropathy has various clinical manifestations, complex pathological changes and different prognosis, more and more scholars believe that IgA nephropathy is not a single dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61K35/74A61K38/17A61K39/39A61K49/00
Inventor 彭文王云满王浩金周慧刘育军殷佩浩李琦姚卫国付文成朱冰冰程蔚蔚吴芳
Owner SHANGHAI PUTUO DISTRICT CENT HOSPITAL
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