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Precise quantization method for nitrogen cycle of lake ecosystem

An ecosystem and nitrogen cycle technology, applied in biological testing, material inspection products, testing water, etc., can solve the problem of difficulty in accurately simulating the nitrogen cycle process, and achieve the effect of reducing the concentration of nutrient salts

Inactive Publication Date: 2014-03-26
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the increasingly serious problem of lake eutrophication and the difficulty of accurately simulating the nitrogen cycle process in different lake ecological restoration methods with existing methods

Method used

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  • Precise quantization method for nitrogen cycle of lake ecosystem
  • Precise quantization method for nitrogen cycle of lake ecosystem
  • Precise quantization method for nitrogen cycle of lake ecosystem

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Small laboratory test, indoor simulation of the nitrogen cycle process between the sediment-water interface, water-air interface, aquatic plants, and nitrogen-removing microorganisms in Meiliang Bay, Taihu Lake. Meiliang Bay is located in the northern part of Taihu Lake, and is the lake area with the worst water quality in Taihu Lake. From 2005 to 2008, the water quality was generally inferior to Class V, and it belongs to the algae-type lake area with serious eutrophication. In-situ collection of Meiliang Bay columnar sediment, overlying water and Elodea.

[0055] The specific experimental steps are as follows:

[0056] (1) Fill the lake sediment from the original position into the bottom of the column from the bottom of the test column according to its original appearance. The thickness of the sediment is 20cm, and adjust it to a suitable height. The collected overlying water was cultivated.

[0057] (2) Select Elodea with a certain amount of biomass and grow vigoro...

Embodiment 2

[0074] Nitrogen cycle research was carried out in Gonghu Bay of Taihu Lake. Gonghu Lake is located in the northeast of Taihu Lake. It used to be one of the areas with better water quality in Taihu Lake. In recent years, with the rapid economic development in the basin and the impact of human activities, nitrogen, phosphorus Nutrient load remains high, water pollution intensifies, and water quality continues to deteriorate. The columnar sediment, overlying water and hornwort algae of Gonghu Lake were collected in situ, and the cycle process of nitrogen in Gonghu Bay of Taihu Lake was simulated indoors at the sediment-water interface, water-air interface, aquatic plants, and nitrogen-removing microorganisms.

[0075] The specific experimental steps are as follows:

[0076] (1) Fill the lake sediment from the original position into the bottom of the column from the bottom of the test column according to its original appearance. The thickness of the sediment is 20cm, and adjust it...

Embodiment 3

[0088] Nitrogen cycle research was carried out in Jinshu Bay of Taihu Lake. The river flows slowly, surrounded by rural domestic sewage discharge, farmland tail water and enterprise sewage discharge, and the water body has relatively high turbidity. In-situ collection of columnar sediment, overlying water, and hydrilla from the river channel was carried out, and the cycle process of nitrogen in the river at the sediment-water interface, water-air interface, aquatic plants, and denitrification microorganisms was simulated indoors.

[0089] The specific experimental steps are as follows:

[0090] (1) Fill the river channel sediment taken from the original position into the bottom of the column from the bottom of the test column according to its original appearance. The thickness of the sediment is 20cm, and adjust it to a suitable height. The collected overlying water was cultivated.

[0091] (2) Select a certain amount of vigorous growth of Hydra verticillium, each submerged p...

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Abstract

A precise quantization method for nitrogen cycle of a lake ecosystem includes 1), applying an isotope pairing method to quantitatively research nitrogen migration and conversion of a sediment-water interface of the lake ecosystem; 2), determining release of nitrogen of a water-gas interface by measuring N2O yield by the aid of a closed chamber-gas chromatograph method; 3), measuring biomass of aquatic plants and nitrogen content in bodies of the aquatic plants by the aid of a Dumas method and determining nitrogen absorbed quantity of the aquatic plants; 4), measuring biomass of microorganisms in the ecosystem by the aid of an MPN (most probable number) method, and measuring the effect of the microorganisms in a nitrogen conversion process and conversion quantity of the microorganisms to the nitrogen; and 5), applying the stable isotope technology to measure assimilation of aquatic animals to the nitrogen, and realizing precise quantization of the nitrogen, namely, measuring a food web of the aquatic animals and a trophic-level structure to determine migration and conversion of the nitrogen in the food web. Procedures of the precise quantization method are realized in the same simulation ecosystem.

Description

technical field [0001] The invention belongs to the technical field of ecological engineering restoration, and in particular relates to a method for accurately quantifying the nitrogen cycle of a lake ecosystem. Background technique [0002] With the rapid development of my country's economy and the acceleration of urban and rural construction, the discharge of pollutants such as nitrogen, phosphorus, and organic matter in areas along rivers and lakes has increased year by year. According to the 2010 China Environmental Status Bulletin, among the 26 state-controlled key lakes (reservoirs), 1 was severely eutrophic, accounting for 3.8%, 2 were moderately eutrophic, accounting for 7.7%, and mildly eutrophic was 7.7%. 11, accounting for 42.3%, the others are mesotrophic, accounting for 46.2%. The main pollution indicators are total nitrogen and total phosphorus. It shows that the overall eutrophication of lake water bodies in the country is relatively serious, the situation i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/48G01N33/18
Inventor 李正魁赵琳周涛吴宁梅王易超叶忠香刘丹丹华蓉
Owner NANJING UNIV
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