Cell secreta production system based on photosensitive gene and cell secreta production method

A technology of cell secretion and production method, applied in the field of cell secretion production system, which can solve the problems of low output and high cost

Inactive Publication Date: 2012-06-27
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In the traditional cell secretion production method, the o

Method used

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  • Cell secreta production system based on photosensitive gene and cell secreta production method
  • Cell secreta production system based on photosensitive gene and cell secreta production method
  • Cell secreta production system based on photosensitive gene and cell secreta production method

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Embodiment Construction

[0035] Many secretion phenomena are closely related to cellular depolarization, mostly due to voltage-gated Ca 2+ channel open, Ca 2+ Influx causes cell secretion. For example, nerve excitation causes the secretion of parotid gland and adrenal medulla cells, and glucose in the plasma promotes the secretion of islet cells, all of which are caused by the depolarization of the cell membrane and the entry of calcium ions into the cells. Some people think that the secretion of hormones also works through calcium ions. The fusion of the cell membrane and the secretory vesicle is also related to the phase transition of the lipid bilayer caused by calcium ions. Some people think that a certain concentration of calcium ions (10 -2 ~10 -1 M) Make the local area in the liquid lipid bilayer produce a crystalline state. In the process, it releases heat while causing the discontinuity of the membrane structure, forming an unstable and highly permeable area, which is conducive to similar...

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Abstract

A cell secreta production system based on a photosensitive gene comprises a bioreactor, a light source and a control device, wherein the bioreactor is used for accommodating a secretory cell expressing the photosensitive gene, the light source is used for carrying out illumination stimulation to the secretory cell in the bioreactor, and the control device is connected with the light source to control the light source to give out light. The cell secreta production system utilizes illumination stimulation to facilitate depolarization of the secretory cell, further causes internal flow of Ca2+, enhances cell secretion function, increases yields of genetic engineering medicines and a monoclonal antibody, and improves production efficiency. In addition, a cell secreta production method is further provided.

Description

【Technical field】 [0001] The invention relates to biotechnology, in particular to a system and method for producing cell secretions. 【Background technique】 [0002] Cell secretion activity is one of the basic processes of biological information transmission and realization of cell functions, involving a series of complex molecular activities and signal transduction processes, secreted substances include various hormones, cytokines, enzymes, neurotransmitters, serum proteins , antibodies and extracellular matrix components, etc. The occurrence of many diseases is closely related to the abnormal content of cell secretion products, such as insulin deficiency or relative deficiency leading to diabetes, congenital hypothyroidism commonly known as cretinism is caused by insufficient secretion of thyroid hormone. Therefore, many diseases are treated by supplementing one or more secretory substances. [0003] Many cell secretions currently used clinically, such as hormones, cytoki...

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Application Information

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IPC IPC(8): C12M1/00C12M1/36C12P1/00
CPCC12M35/02C12M41/06
Inventor 张奕王立平卢恬
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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