Directional emission fluorescence imaging detection device

A fluorescence imaging and detection device technology, which is applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of inability to quickly locate the region of interest, expensive, expensive devices, etc., to achieve easy implementation and promotion, rapid positioning, The effect of simple structure
CN102539404BInactive Publication Date: 2014-07-02XIAMEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
XIAMEN UNIV
Publication Date
2014-07-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a fluorescence imaging detection device, and particularly provides a directional emission fluorescence imaging detection device, which is provided with an optical laser beam-expanding system, a sample system and a detecting system, wherein the optical laser beam-expanding system is provided with a laser light source, a microobjective, a needle hole, a plano-convex lens, an attenuator and a rectangular diaphragm, the excitation light emitted by the laser light source sequentially passes by the microobjective, the needle hole and the plano-convex lens for beam expanding and collimation to form parallel light beams, and the parallel light beams sequentially pass by the attenuator and the diaphragm to obtain light spots; the sample system is provided with a rotating stage, a sample rack, a prism and an optical quartz substrate, the prism is placed on the sample rack and fixed at the center of the rotating stage, a nanoscale metallic film is arranged on the surface of the quartz substrate, a fluorescence sensing film is arranged on the surface of the metallic film, and solution is coated between the quartz substrate and the prism; and the detecting system is provided with a CCD rotating arm, a CCD receiver and an optical filter, the CCD rotating arm is fixed on the stage board of the rotating stage, the CCD rotating arm is connected onto the stage board of the rotating stage, and the CCD receiver used for collecting images and the optical filter are arranged on the CCD rotating arm.
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Description

technical field

[0001] The invention relates to a fluorescence imaging detection device, in particular to a prism-type surface plasmon coupling directional emission fluorescence imaging detection device. Background technique

[0002] Due to the increasing application of fluorescence analysis technology in life science fields such as immune analysis, gene expression, drug screening, biosensing, and cell imaging, the research on new fluorescence technology and instruments has great significance and broad development prospects. Surface Plasmon Coupled Directional Emission (SPCDE) is a new fluorescence analysis method proposed recently. After the coupling of surface plasmons generated by free electrons, the SPCDE angle corresponding to the fluorescence emission wavelength (ie θ SPCDE ) into the prism matrix radiation. As a new fluorescence technology, SPCDE not only has the advantages of metal surface fluorescence such as enhanced fluorescence intensity, quantum yield and phot...

Claims

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