Method for detecting hydrocortisone and special enzyme-linked immunosorbent assay kit thereof
An enzyme-linked immunosorbent reagent, hydrocortisone technology, applied in the direction of measuring devices, cells modified by introducing foreign genetic material, instruments, etc. High personnel requirements are required to achieve the effect of convenient and easy inspection methods, shortened inspection time, and simplified steps
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] Example 1, the kit and its detection method using hydrocortisone hapten and carrier protein conjugate as coating source
[0029] 1. The detection principle of the kit with hydrocortisone hapten and carrier protein conjugate as the coating source is as follows:
[0030] When pre-coating hydrocortisone-conjugated antigen on the microwell strip of the microtiter plate, after adding the sample solution or standard solution, add the hydrocortisone-specific antibody solution, and the hydrocortisone drug and enzyme in the sample The hydrocortisone-coupled antigen coated on the target plate competes with the hydrocortisone-specific antibody, the enzyme-labeled anti-antibody is added for amplification, the color is developed with a chromogenic solution, and the absorbance value of the sample is correlated with the content of the hydrocortisone drug It is negatively correlated, and the content of hydrocortisone in the sample can be obtained by comparing with the standard curve. A...
Embodiment 2
[0098] Embodiment 2, test kit precision, accuracy and shelf life test
[0099] 1. Standard product precision test:
[0100] From the three different batches of kits prepared in step 3 of Example 1, 10 kits were respectively extracted, and 20 microwells were respectively extracted from the enzyme-linked plate of each kit, and measured Calculate the coefficient of variation from the absorbance value of 0.3 μg / L hydrocortisone standard solution. The results are shown in Table 1.
[0101] Table 1. Standard repeatability test (CV%)
[0102]
[0103] Experiments show that the coefficients of variation of standard products measured 10 times by each batch of kits are all between 3.8% and 9.5%, which meets the requirement that the precision is less than or equal to 20%.
[0104] 2. Sample precision and accuracy test
[0105] (1) Sample precision test:
[0106] Add hydrocortisone with a final concentration of 5 μg / L to feed and milk without hydrocortisone, and then perform sampl...
PUM
| Property | Measurement | Unit |
|---|---|---|
| volume | aaaaa | aaaaa |
| volume | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More