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Endophytic fungi CEF08111 of cotton and application thereof in prevention and treatment of cotton verticillium wilt

A technology of CEF08111 and cotton verticillium wilt, applied in the field of phytopathology, can solve problems such as poor control effect of cotton verticillium wilt, and achieve the effects of being conducive to mass reproduction and popularization and application, good control effect and significant control effect

Active Publication Date: 2013-10-09
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the inventors of the present invention propose and complete the present invention in order to solve the problem that the existing control effect of antagonistic microorganisms to control cotton verticillium wilt is poor.

Method used

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  • Endophytic fungi CEF08111 of cotton and application thereof in prevention and treatment of cotton verticillium wilt
  • Endophytic fungi CEF08111 of cotton and application thereof in prevention and treatment of cotton verticillium wilt
  • Endophytic fungi CEF08111 of cotton and application thereof in prevention and treatment of cotton verticillium wilt

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1 Isolation of bacterial strains

[0020] (1) Sample collection

[0021] In July-August, in cotton areas such as Hubei, Sichuan, and Jiangsu in the south, it is best to choose healthy cotton plants after the rain, and take 15cm from the middle and upper parts of the main stem and fruit branches.

[0022] (2) Isolation of strains

[0023] First, the collected samples (cotton stalks) are washed with clear water, peeled off, placed in a sterilized petri dish with a diameter of 9 cm, and sterilized with 75% alcohol for 1 min. Then disinfect with 5.0% sodium hypochlorite for 60 seconds, and wash with sterilized water for 3 times, each time not less than 3 minutes. Then use sterilized absorbent paper to dry the water on the surface, cut into 0.4cm long pieces with sterilized scissors, place them in a 9cm petri dish, and incubate at 25°C. Use the last cleaning solution as a control to check whether the disinfection is thorough. Check the growth of endophytic fungi ...

Embodiment 2

[0043] Example 2 Control Effect of CEF08111 on Cotton Verticillium Wilt

[0044] 1. Test method The disease-resistant variety Lumianyan 28 and the susceptible variety Jimian 11 were used as the test varieties, and the vermiculite sandy soil was used to quantitatively dip in the bacterial solution. Mix sterilized vermiculite and sand at a ratio of 6:4, make a bottomless paper pot with a diameter of 6 cm, place it in a plastic tray of 45 cm × 35 cm, sow 8 cotton seeds in each pot, and keep 5 seedlings after emergence, 15 for each variety. bowls, every 5 bowls is a repetition, 3 repetitions. After the cotton is sown, put the plastic tray in the greenhouse, wait for the cotton seedling to grow a true leaf, inoculate 10ml of the spore suspension according to the test settings, and the spore concentration is 1×10 7 spores / ml. The inoculation method is as follows: pour 10ml of spore suspension into a petri dish with a diameter of 10cm, take out the paper bowl from the plastic dish ...

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Abstract

The invention relates to phytopathology, in particular to endophytic fungi CEF08111 of cotton and an application of the endophytic fungi CEF08111 in prevention and treatment of cotton verticillium wilt. According to the invention, a strain CEF08111 (Gibellulopsis nigrescens) with the collection number of CGMCC No. 5610, which has a significant control effect on the cotton verticillium wilt, is obtained from the endophytic fungi of the cotton by separation, screening, identification and biological assay in a green house. The CEF08111 can be cultured on an artificial culture medium, is easy to obtain and culture, and is conductive to factory production, popularization and application. The CEF08111 has a good control effect on the verticillium wilt; the CEF08111 is firstly inoculated, and verticillium dahliae is inoculated 4 days later, so that the prevention and treatment effect on the cotton verticillium wilt reaches 97.2 percent and the control effect on the verticillium wilt is remarkable; and compared with bacillus subtilis which is more applied to production, the CEF08111 has the advantage that the prevention and treatment effect is improved by about 50 percent, and is safe to people, animals, insects and crops and is environment-friendly.

Description

technical field [0001] The invention relates to plant pathology, in particular to cotton endophytic fungus CEF08111 and its application in the control of cotton verticillium wilt. Background technique [0002] Verticillium dahliae caused by Verticillium dahliae is one of the important diseases of cotton worldwide. Due to the lack of truly effective disease-resistant varieties or the resistance of disease-resistant varieties is easily lost, the most economical and effective control measures cannot play its leading role. Since the 1990s, the disease has become a disaster in major cotton areas in my country, with an annual occurrence area of ​​about 3 million hectares and a loss of about 100 million kilograms of lint, which has become one of the main limiting factors for the development of cotton production. Due to the difficulty of crop rotation, the lack of disease-resistant varieties, the poor control effect of chemical agents, and the lack of pesticides with good control e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 朱荷琴冯自力李志芳师勇强赵丽红
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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