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System and method for continuous, asynchronous autofocus of optical instruments

A technology of automatic focusing and optical instruments, which is applied to the focusing device of cameras, focusing devices of projectors, instruments, etc., and can solve problems such as distance fluctuations of imaging optical components

Active Publication Date: 2012-07-18
LEICA MICROSYSTEMS CMS GMBH
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Problems solved by technology

The focus of an optical instrument may change over time due to, for example, thermal and electromechanical instabilities, and the stage translates in the x-y plane as the sample is scanned or data is collected from the sample over a period of time, even with very precise electromechanical microscopes. The object stage may also fluctuate in distance relative to the imaging optics

Method used

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  • System and method for continuous, asynchronous autofocus of optical instruments
  • System and method for continuous, asynchronous autofocus of optical instruments
  • System and method for continuous, asynchronous autofocus of optical instruments

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Embodiment Construction

[0021] Figure 1A-Figure 1C The diagram shows the light path in a fluorescence microscope. The optical path and the fluorescence microscope containing the optical path are used as the context for describing one embodiment of the present invention. However, as also mentioned below, in many cases, the methods and systems of the present invention can be incorporated into a wide range of different types by adjusting various parameters and configurations of the embodiments of the present invention for use in specific applications. In optical instruments.

[0022] The optical path of a fluorescence microscope includes an excitation light source 102 (usually a visible light or UV light laser), a polychroic mirror 104 that reflects the excitation light 106 to an objective lens or lens 108, and the objective lens or lens 108 focuses the excitation light on a mechanical load platform. A point 110 in the sample supported on the distal side of the cover glass 112 on 114, and the mechanical l...

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Abstract

Embodiments of the present invention are directed to autofocus subsystems within optical instruments that continuously monitor the focus of the optical instruments and adjust distances within the optical instrument along the optical axis in order to maintain a precise and stable optical-instrument focus at a particular point or surface on, within, or near a sample. Certain embodiments of the present invention operate asynchronously with respect to operation of other components and subsystems of the optical instrument in which they are embedded.

Description

[0001] Cross reference to related applications [0002] This application requires the rights of provisional application number 61 / 256,242 on October 29, 2009 and provisional application number 61 / 267,353 filed on December 7, 2009. Technical field [0003] The present invention relates to an optical instrument, and more specifically, to a continuous, asynchronously operated auto-focusing sub-system in an optical instrument that keeps an objective lens and an imaging object on, within, or near points or surfaces along the imaging object. Constant distance of the optical axis. Background technique [0004] For hundreds of years, when optical microscopes and other optical-based methods were used in many fields of human activities from scientific research to warfare, the emergence of microprocessors, modern computing, and molecular biology prompted new optical instruments and optical imaging The further accelerated development of technology. For example, the fluorescent labeling of pro...

Claims

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Application Information

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IPC IPC(8): G02B7/28G02B26/00G03B3/10G03B13/36G02B21/00
CPCG02B21/245G02B21/16
Inventor J·库珀P·古德文
Owner LEICA MICROSYSTEMS CMS GMBH
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