Glabrous sarcandra herb polysaccharide and preparation method and application thereof
A technology of sarcophagia and polysaccharides, which is applied in the field of preparation of sarcophagia polysaccharides, can solve the problem that the substance basis of the drug effect is not very clear, the chemical composition of small molecules is difficult to explain the clinical efficacy of sarcomeres, and the sugar content of water-soluble crude polysaccharides is low And other issues
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Embodiment 1
[0029] Example 1: Preparation Method 1 of Crude Polysaccharide of Sargassum Rhizoma Sargassum
[0030] Weigh 100g of the whole herb of S. chinensis that has been crushed and passed through a 80-mesh sieve into an extraction container, add 5 times the volume of ethanol, reflux and degrease for 3 hours, remove the supernatant with 4 layers of gauze, make up the residue with deionized water to 1000ml, and place in a 70°C water bath Extract for 1 hour with intermittent stirring. After collecting the extract, add deionized water to the residue to 1000ml, extract in a water bath at 70°C for 1 hour, and stir intermittently. Gauze is filtered to remove residue, the combined extracts are concentrated to 20ml, centrifuged at 4000r.p.m to remove the precipitate, added 1 times of ethanol to precipitate, placed at 4°C for 24 hours, centrifuged to remove the supernatant, washed 3 times with absolute ethanol, and vacuum-dried to obtain the present invention. The product is sarcophagus feng ...
Embodiment 2
[0031] Example 2: Preparation Method 2 of Crude Polysaccharide of Sargassum Rhizoma Sargassum
[0032]Weigh 100g of the whole herb of S. chinensis that has been crushed and passed through a 120-mesh sieve into an extraction container, add 20 times the volume of ethanol, reflux and degrease for 3 hours, remove the supernatant with 4 layers of gauze, make up the residue with deionized water to 3000ml, and place in a 90°C water bath Extract for 3 hours. Stir intermittently, after collecting the extract, make up the residue with deionized water to 3000ml, extract in a water bath at 90°C for 3 hours, and stir intermittently. Gauze is filtered to remove residue, the combined extract is concentrated to 20ml, centrifuged at 4000r.p.m to remove the precipitate, added 6 times of ethanol to precipitate, placed at 4°C for 24 hours, centrifuged to remove the supernatant, washed 3 times with absolute ethanol, and vacuum-dried to obtain the present invention. The product is sarcophagus feng...
Embodiment 3
[0033] Example 3: Preparation of Uniform Sargassum Polysaccharide
[0034] Take 200 mg of SGP crude polysaccharide (SGP), fully dissolve it in deionized water, and put it on a well-balanced DEAE-52 ion-exchange column, the specification is (2.6×30cm), the balance liquid is deionized water, and the loading volume is 10ml , first eluted with 300ml deionized water, then eluted with 0-2mol / L NaCl solution, collected in sections, tracked and detected by sulfuric acid phenol method, and combined the same components. The water-washed fraction of the polysaccharide of Sargassum obtained through ion-exchange column separation was subjected to Sephacryl S-400 molecular sieve column chromatography and eluted with deionized water. The column size is (1.0×100cm), collected in sections, tracked and detected by the sulfuric acid phenol method, and combined the same components. Freeze-dry to obtain the fine polysaccharide of sargassum polysaccharide (code name: SGP-1).
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