A detection method of Yangjinhua Capsule, a traditional Chinese medicine for treating psoriasis

A detection method and the technology of Yangjinhua, which are applied in measurement devices, color/spectral characteristic measurement, instruments, etc., can solve problems such as inability to cure and limited curative effect.

Inactive Publication Date: 2017-06-16
匡海学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many types and quantities of drugs for these systemic and local treatments, their curative effects are very limited, and long-term treatment is often required, but it is still repeated or even lifelong and cannot be cured.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A detection method of Chinese medicine Yangjinhua capsules for treating psoriasis. The method is divided into eight steps: the first step is to identify 3-O-β-D- The method of glucopyranosyl (1→2) glucopyranosyl-7-O-α-L-rhamnopyranosyl kaempferol, the second step is to identify the effective part of the foreign Chinese medicine Jinhua Capsule by TLC Central ginseng C is 5α, 12β, 27-trihydroxy-6α, 7α-epoxy-(20R, 22R)-1-keto-withan-2,24-dienolactone-27-O-β -D-glucopyranoside, the third step is the method of thin layer chromatography to identify the alkaloid components in the effective part of the traditional Chinese medicine Yangjinhua Capsules, the fourth step is to determine the effective parts of the traditional Chinese medicine Yangjinhua Capsules by ultraviolet spectrophotometry The method for the total flavonoid content, the fifth step is the method for determining the total steroid content in the effective part of the traditional Chinese medicine Yangjinhua capsule...

Embodiment 2

[0040] The detection method of the Chinese medicine Yangjinhua Capsules for the treatment of psoriasis described in Example 1, the thin-layer chromatographic identification of 3-O-β-D-glucopyranosyl (1 →2) The method of glucopyranosyl-7-O-α-L-rhamnopyranosyl kaempferol,

[0041] First, take 1 capsule of the traditional Chinese medicine Yangjinhua capsule, add 2-3ml of methanol, seal it up, extract it by ultrasonic for 30 minutes, let it stand still, filter, and dilute the filtrate to 4-6ml as the test solution, and then take another 3-3ml of the reference substance. O-β-D-glucopyranosyl (1→2)glucopyranosyl-7-O-α-L-rhamnopyranosyl kaempferol 2mg, add methanol to 9-11ml volumetric flask According to the thin-layer chromatography test, absorb the test solution and the reference solution, respectively point on the same polyamide film plate, with methanol-water-glacial acetic acid 4:6:0.1 as the developer After the color development of 5% aluminum trichloride ethanol solution, che...

Embodiment 3

[0043] The detection method of the Chinese medicine Yangjinhua Capsules for the treatment of psoriasis described in Example 1, the thin-layer chromatographic identification of the effective part of the traditional Chinese medicine Yangjinhua Capsules, i.e. 5α, 12β, 27-trihydroxy- 6α,7α-epoxy-(20R,22R)-1-keto-withan-2,24-dienolactone-27-O-β-D-glucopyranoside method,

[0044] Take 20 capsules of the traditional Chinese medicine Yang Jinhua, add 90-110ml of methanol, seal it tightly, extract it by ultrasonic for 25-35 minutes, let it stand still, filter it, and transfer the recovered filtrate with 2-10ml of water to make a water transfer solution; The water transfer solution is passed through D941 macroporous weakly basic acrylic anion exchange resin, and the water eluate is collected to dryness. It is transferred with methanol to a constant volume in a 9-11ml volumetric flask as the test solution; Anthoside C5α, 12β, 27-trihydroxy-6α, 7α-epoxy-(20R, 22R)-1-keto-withan-2,24-dieno...

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Abstract

The invention provides a quality control method of a Chinese medicinal hindu datura flower capsule for treating psoriasis. The psoriasis is a skin disease with complicated and unclarified pathogenesis. The method comprises the following steps: performing thin layer chromatography to determine whether the effective position of the hindu datura flower capsule contains 3-O-beta-D-glucopyranose, hindu datura glucoside C, 5 alpha,12 beta,27-trihydroxy-6alpha,7alpha-epoxy, 20R,22R, -1-ketone-witha-2,24-diene lactone-27-O-beta-D-glucopyranoside and alkaloids or not; detecting the contents of total flavonoids and total steroids in the effective position of the hindu datura flower capsule by using an ultraviolet spectroscopy method; detecting the contents of the 3-O-beta-D-glucopyranose (1->2) glucopyranose-7-O-alpha-L-rhamnopyranose tiliroside and the hindu datura glucoside C by using a reversed phase highperformance liquid chromatography method; and establishing limit test of alkaloids in the effective position of the hindu datura flower capsule. The quality control method is used in quality control of the hindu datura flower capsule.

Description

[0001] Technical field: [0002] The invention relates to a detection method of the traditional Chinese medicine yangjinhua capsule for treating psoriasis. [0003] Background technique: [0004] Psoriasis is a common, lingering, difficult-to-cure, easy-to-relapse skin disease with complex pathogenesis that has not yet been elucidated. Since ancient times, doctors have regarded psoriasis as an intractable disease, so there is a saying that "if you don't cure ringworm, you will lose face if you treat ringworm". Although the incidence rate of this disease varies greatly around the world, the prevalence rate of the natural population is between 0.1% and 3%. And the prevalence rate is higher, all brings great misery to patient's body and mind. At present, the drugs for the treatment of psoriasis at home and abroad mainly include corticosteroids, immunosuppressants, retinoic acid, calcipotriol, anthralin, tar and so on. Although there are many kinds and quantities of medicines fo...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): G01N30/90G01N30/02G01N30/06G01N21/31
Inventor匡海学杨炳友王秋红王知斌孙海姣
Owner匡海学