Method for identifying Alternaria altemata (Fr.) Keissler resistance of pear germplasm
A pear black spot and anti-black spot technology is applied in horticultural methods, botanical equipment and methods, biochemical equipment and methods, etc., and can solve the problems of pathogenic bacteria resistance to chemical agents, environmental pollution, human health, harm and other problems, To achieve the effect of simple operation, easy method and accurate identification results
Inactive Publication Date: 2014-03-12
INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI
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Problems solved by technology
The current method of preventing and treating pear black spot is to use chemical agents such as thiophanate-methyl, mancozeb and difenoconazole. If these chemical agents are used for a long time, if they are used improperly, it will inevitably cause pathogenic bacteria to develop resistance to the chemical agents and cause pollution. environmental and human health hazards
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[0054] A method for identifying anti-black spot of pear germplasm, the steps are:
[0055] 1. Preparation of black spot pathogen:
[0056] (1) Preparation of medium:
[0057] Potato medium (PSA): 200g potato (peeled), 20g agar powder, 20g sucrose, 1000mL water. The preparation method is as follows: Peel the potatoes and cut them into about 2cm 2 Small pieces, boiled for 0.5h, pay attention to stirring with a glass rod to prevent paste. Then filter with double-layer gauze, take the filtrate and add agar powder, add sucrose after dissolving, and make up water to 1000mL, pH7.0. (2) Isolation and identification of pear black spot disease:
[0058] For the collected leaves or fruits with pear black spot symptoms, tissue isolation, monospore purification, cultivation, and preservation were performed with reference to "Experimental Technology of Microbiology" edited by Du Lianxiang and Lu Fuping in 2005. Inoculate the isolated strains on young pear leaves to observe whether typi...
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The invention discloses a method for identifying Alternaria altemata (Fr.) Keissler resistance of pear germplasm, which comprises the following steps: (1) preparing Alternaria altemata (Fr.) Keissler pathogen, and mainly performing culture medium preparation, Alternaria altemata (Fr.) Keissler separation identification, Alternaria altemata (Fr.) Keissler pathogen transfer and Alternaria altemata (Fr.) Keissler pathogen activation and enlarging culture; (2) preparing a Alternaria altemata (Fr.) Keissler resistance material, and mainly performing pear variety grafting and pear grafting variety transplanting; (3) performing artificial inoculation identification, and mainly utilizing Alternaria altemata (Fr.) Keissler pathogen spore suspension to perform spraying inoculation to grafted seedling blades of an annual pear variety; and (4) performing operation of disease investigation and grading standard. The method is practical and simple and convenient to operate, and the Alternaria altemata (Fr.) Keissler resistance of the pear variety can be identified rapidly and accurately. By means of the method, the Alternaria altemata (Fr.) Keissler resistance of the pear variety in scientific research, breeding and production of pears is identified rapidly.
Description
technical field [0001] The invention belongs to the technical field of fruit trees, and more specifically relates to a method for identification of pear germplasm resistance to black spot disease. The method can effectively solve the problem of fast identification of pear variety resistance to black spot disease in pear scientific research, breeding and production. This method is suitable for scientific research units, companies and individuals engaged in pear scientific research, breeding and production. Background technique [0002] Pear belongs to Rosaceae (Rosaceae) Apple subfamily (Maloideae) Pyrus (Pyrus) plant, is the deciduous fruit tree of Qiaoben, is one of three major cultivated fruit trees in my country, and also occupies a pivotal position in the world. According to the statistics of China Yearbook, in 2010, the pear planting area in my country was 10.631 million hectares, and the output was 15.0571 million tons, ranking first in the world. Pear production has ...
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IPC IPC(8): A01G7/06A01G17/00C12Q1/18
Inventor 胡红菊杨晓平陈启亮田瑞张靖国
Owner INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI