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Method and device for preparing acetonebutanol by utilizing adsorption carrier fermentation

An acetone-butanol and acetone-butanol fermentation technology, which is applied in the field of fermentation engineering, can solve the problems of affecting the permeability of polymer substrates, complex immobilization methods, and increasing the probability of bacterial infection, so as to improve the composition ratio and increase the contact ratio. Surface, the effect of improving production capacity

Inactive Publication Date: 2014-04-30
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
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  • Description
  • Claims
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Problems solved by technology

Patent 87103534 discloses a butanol-producing Clostridium liquid in the exponential growth phase to make immobilized cells through the adsorption of ceramic rings; patent 200910076955.4 discloses a butanol-producing Clostridium liquid in the exponential growth phase, The method of making immobilized cells by straw adsorption, using these patented methods, the cell wall, cell membrane and carrier all have a diffusion limiting effect during the fermentation process, and the size of the pores formed by the carrier will also affect the permeability of the polymer substrate. The container realizes cell immobilization and product fermentation respectively
Patent 201010559493.4 discloses a method for the continuous production of bio-butanol coupled with fixed-bed fermentation separation, but the immobilization method of this patent is relatively complicated, requiring the addition of a packed bed reactor and the circulation of the fermentation broth between the bioreactor and the packed bed , increasing the production cost and increasing the chance of contamination

Method used

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  • Method and device for preparing acetonebutanol by utilizing adsorption carrier fermentation
  • Method and device for preparing acetonebutanol by utilizing adsorption carrier fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Using 80g / L glucose as the substrate and steam explosion-delignification pretreated corn stalks as the carrier, the culture medium was sterilized, inoculated with Clostridium acetobutylicum ATCC 824, and fermented for 72 hours to fully utilize the glucose. After fermentation, the discharge port is opened to obtain a fermentation liquid. Compared with the control liquid fermentation experiment, the total solvent production was increased by 57% in the adsorption carrier fermentation.

Embodiment 2

[0026] With 54g / L xylose as the substrate and non-woven fabric as the carrier, after the medium was sterilized, Clostridium acetobutylicum ATCC 824 was inoculated and fermented for 72 hours to fully utilize xylose. After fermentation, the discharge port is opened to separate the fermentation broth and the adsorption carrier. Compared with the control liquid fermentation experiment, the total solvent production was increased by 27% in the adsorption carrier fermentation.

Embodiment 3

[0028] With 60g / L glucose as the substrate and polyurethane foam as the carrier, Clostridium acetobutylicum ATCC 824 was inoculated after sterilization and fermented for 72 hours to fully utilize glucose. After fermentation, the discharge port is opened to obtain a fermentation liquid. Compared with the control liquid fermentation experiment, the total solvent production was increased by 39% in the adsorption carrier fermentation.

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Abstract

The invention provides a method and a device for preparing acetonebutanol by utilizing adsorption carrier fermentation. The method comprises the following steps of: selecting a non-toxic carrier with good water absorptivity, stable property and a porous structure and uniformly mixing the non-toxic carrier with an acetonebutanol liquid medium in a certain proportion; and after inoculation, performing adsorption carrier fermentation. A traditional fermentation tank is utilized as a fermentation device and is internally provided with one or multiple porous adsorption carrier drums so as to realize solid-liquid separation; and after fermentation is finished, the direct separation of the fermentation liquid from the carrier can be realized by virtue of the gravity action. The adsorption carrier fermentation method provided by the invention can be used for improving the solvent yield of acetone-butanol fermentation and reducing the relative content of by-product ethanol; and the carrier can be reused.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method and a device for applying an adsorption carrier to acetone-butanol fermentation. Background technique [0002] Butanol is an important organic chemical raw material, which can be used as a solvent for paints and surface coatings, and as a new type of biofuel with great potential. Compared with methanol and ethanol, butanol has good water insolubility and low vapor High pressure, high calorific value, etc., can achieve a higher mixing ratio with gasoline, and is more suitable for use in existing fuel supply and distribution systems. [0003] Since the production of butanol by fermentation has the characteristics of renewable raw materials and no dependence on petroleum, the production of butanol by fermentation has gradually become one of the research hotspots of bioenergy. In recent years, bio-butanol production facilities have increased yea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/28C12P7/16C12M1/00
CPCC12M21/12C12M45/04
Inventor 陈洪章贺芹
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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