Method for regulating and controlling mRNA (messenger Ribonucleic Acid) level of zebra fish vitellogenin

Abstract

The invention discloses a method for regulating and controlling the mRNA (messenger Ribonucleic Acid) level of zebra fish vitellogenin. The method is used for checking the toxicity or interference for aquatic organisms caused by low-concentration estrogen disruptors. The method comprises the following steps of: firstly, respectively extracting total RNA of livers of the aquatic organisms to be detected and a control aquatic organism; secondly, quantifying the mRNA expression level of VTG1 (Vitellogenin 1) and/or VTG3 of the vitellogenin of the extracted total RNA of the livers; and thirdly, if the mRNA expression level of the VTG1 and/or VTG3 of the vitellogenin of a sample to be detected is higher than the expression level of the VTG1mRNA or VTG3mRNA of the vitellogenin of a control sample, showing that the endocrine secretion in the aquatic organisms to be detected is poisoned or interfered by the low-concentration estrogen disruptors. According to the method disclosed by the invention, whether the aquatic organisms are poisoned or interfered by the low-concentration estrogen disruptors in an external environment or not can be accurately and sensitively detected; and the method has the advantages of favorable sensitivity, high accuracy and the like and can be applied to the field of detection of estrogen disruptors for the aquatic organisms.

Description

technical field [0001] The invention relates to a method for testing the toxicity or interference of organic pollutants to aquatic organisms, in particular to a method for testing whether the endocrine of aquatic organisms is poisoned or interfered by low-concentration estrogen-like disruptors in the exogenous environment. The field of inspection of biological estrogen disruptors. Background technique [0002] The chemical pollutants in the ecological environment interfere with the endocrine function of humans and wild animals, thereby exerting various effects on individual reproduction, development, and sexual behavior, and even affecting the survival and reproduction of humans (Tilghman S L, Nierth-Simpson E N, Wallace R, et al. Environmental hormones: Multiple pathways for response may lead to multiple disease outcomes [J]. Steroids, 2010, 75(8-9): 520-523). Therefore, international organizations and developed countries implement mandatory management on monitoring the ha...

AI Technical Summary

Problems solved by technology

When aquatic organisms are poisoned or polluted by low concentrations of estrogen-like disruptors, neither the external morphological characteristics nor the internal physiological indicators will show obvious changes. In addition, the existing methods for evaluating estrogen-like interference The biomarkers for the endocrine dis

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