Application of miRNA (Micro-Ribonucleic Acid) expression profile in predicting sensibilities of lung cancer patients to radiotherapy

A radiotherapy and expression profiling technology is applied in the application field of miRNA in predicting the sensitivity of lung cancer patients to radiotherapy, achieving the effects of strong clinical practicability, reduction of over-treatment and rich sources.

Inactive Publication Date: 2010-09-08
INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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Problems solved by technology

At present, most of the studies on the relationship between microRNA and tumor focus on the relationship between tumor occurrence, development and prognosis. There are no research reports on the relationship between miRNA expression profile and tumor radiotherapy sensitivity at home and abroad, especially the role of miRNA expression profile in predicting the response of lung cancer patients. Applications in Radiation Therapy Sensitivity

Method used

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  • Application of miRNA (Micro-Ribonucleic Acid) expression profile in predicting sensibilities of lung cancer patients to radiotherapy
  • Application of miRNA (Micro-Ribonucleic Acid) expression profile in predicting sensibilities of lung cancer patients to radiotherapy
  • Application of miRNA (Micro-Ribonucleic Acid) expression profile in predicting sensibilities of lung cancer patients to radiotherapy

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Embodiment 1

[0027] Patients and specimens: 15 cases of lung cancer patients who were sensitive to radiotherapy and 15 patients who were resistant to radiotherapy were selected from the lung cancer patients who received postoperative radiotherapy in the cooperative hospital. 5ml of peripheral blood was drawn, anticoagulated, and stored in a -80°C low-temperature refrigerator.

[0028] 2. Separation of peripheral blood lymphocytes and extraction of total RNA

[0029] 1) Add lymphocyte separation medium (Shanghai Second Reagent Factory) into the centrifuge tube.

[0030] 2) Take EDTA anticoagulated venous blood and mix it well with an equal amount of normal saline, and use a dropper to slowly superimpose on the layered liquid surface along the tube wall, keeping a clear interface. Centrifuge horizontally at 2000 rpm for 20 minutes.

[0031] 3) After centrifugation, the inside of the tube is divided into three layers, the upper layer is plasma and saline, and the lower layer is mainly red bl...

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Abstract

The invention discloses application of a miRNA (Micro-Ribonucleic Acid) expression profile in predicting the sensibilities of lung cancer patients to the radiotherapy. A preparation method for the invention comprises the following steps of: selecting lung cancer patients who are clinically unusually sensitive and highly tolerant to the radiotherapy, drawing 5ml of peripheral blood, and extracting total RNA by using a TRIZOL kit (Invitrogen); then hybridizing by using a MicroRNA expression profile chip; carrying out data processing, and analyzing the difference of MicroRNA expression profiles of radiotherapy sensitive and non-sensitive patients by using a cluster analysis method; and finally, checking a result by using a Real-time PCR (Polymerase Chain Reaction) method. The inventor finds that an expression profile consisting of 12 miRNA can regulate and control the expression of genes relevant to tumor radiosensitivity and be used for predicting the sensitivity of the lung cancer patients to the radiotherapy and providing an experimental foundation of an early period for the individualized radiotherapy of lung cancer.

Description

technical field [0001] The invention belongs to the technical field of medical and biological inventions, and relates to a research method for predicting radiosensitivity of tumors, more specifically, the application of miRNA in predicting sensitivity of lung cancer patients to radiotherapy. Background technique: [0002] Lung cancer occurs in the bronchial mucosal epithelium, also known as bronchial lung cancer. Lung cancer generally refers to cancers of the lung parenchyma, usually excluding other tumors such as mesothelioma of pleural origin, or other malignant tumors such as carcinoid, malignant lymphoma, or metastases from other tumors. source of tumor. Therefore, what we call lung cancer refers to malignant tumors from bronchial or bronchiolar epithelial cells, accounting for 90-95% of lung parenchymal malignant tumors. Lung cancer is currently the number one cause of cancer death in the world. In 1995, 600,000 people died of lung cancer in the world, and the number ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 王小春田静田丽丽吴海良孟爱民赵永成王月英李德冠路璐吴红英张恒
Owner INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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