3results about How to "Increased sequencing depth" patented technology

The invention discloses a congenital agomphosis gene detection panel and application thereof, and belongs to the technical field of gene detection. The congenital odontia gene detection panel comprises probes for detecting or hybridizing 104 congenital odontia related genes shown in a capture table 1, a sample DNA and the panel are subjected to solution / chip hybridization, sequencing is carried out after DNA enrichment of a target area is completed, harmful sites or genes related to congenital odontia are screened / predicted according to a determination result, and the congenital odontia gene detection panel is used for detecting or hybridizing the 104 congenital odontia related genes. The expansibility, speed and resolution required for evaluating the target gene can be met, multiple genes in multiple samples can be evaluated at the same time, time is saved, and cost is reduced. Besides, the panel sequencing is high in coverage rate, strong in pertinence, high in detection sensitivity, higher in effectiveness, easier to manage and capable of more accurately, comprehensively and effectively detecting the related genes of the congenital odontoprisis and mutation conditions of the related genes, and accurate diagnosis and risk assessment of the congenital odontoprisis are achieved.

This invention discloses a set of primers and a high-throughput sequencing method for goose mitochondrial genome sequencing. The method comprises (1) extracting genomic DNA from the goose to be tested; (2) performing PCR amplification using the primer set described in this invention; (3) performing high-throughput sequencing; and (4) obtaining the mutation type and haplotype through detection. This invention provides the application of the PCR primers or the goose mitochondrial genome high-throughput sequencing method described in this invention in detecting different mutation types or haplotypes.

This invention discloses a method for multiplex amplification of the duck mitochondrial genome, the method comprising: (1) extracting genomic DNA from the duck to be tested; (2) performing PCR amplification using the primer set described in this invention; (3) performing high-throughput sequencing; and (4) obtaining the mutation type and haplotype through detection. This invention provides the application of the PCR primers or the duck mitochondrial genome multiplex amplification method described in this invention in detecting different mutation types or haplotypes.