Gene chip for high-depth sequencing of gene mutation, preparation method and applications thereof

Abstract

The invention discloses a gene chip for high-depth sequencing of gene mutation, a preparation method and applications thereof. The preparation method of the gene chip comprises the following steps: constructing capture interval libraries by combining a gene mutation online database and a local database according to different mutation types; and designing a gene chip by the capture interval library, so that the capture quality and the capture efficiency are improved, and high-depth sequencing is realized, wherein the mutation types comprise at least one selected from mononucleotide variation, insertion and deletion mutation, copy number mutation and structural variation, and the capture interval libraries comprise a mononucleotide variation and insertion and deletion mutation capture interval library, a copy number mutation capture interval library and a structural variation capture interval library. According to the preparation method of the gene chip, mutant people can be covered to the maximum extent, cancers and related genes thereof can be covered to the maximum extent, and the gene chip suitable for Chinese people is designed. The gene chip disclosed by the invention is high in sequencing depth, and is particularly suitable for detecting extremely trace circulating DNA in blood.

Description

technical field

[0001] The present application relates to the field of nucleic acid sequencing, in particular to a gene chip for high-depth sequencing of gene mutations and its preparation method and application. Background technique

[0002] Tumors are one of the diseases that seriously threaten human health, and tumors are highly heterogeneous, that is, tumors in different parts have different characteristics. Even for tumors in the same part, the treatment effect varies from person to person. In order to improve the effect of clinical tumor treatment, it is imperative to carry out individualized tumor treatment based on human factors and time, considering the individual differences of tumors and the characteristics of tumor cell development and evolution. With the development of the field of cancer cognition and the advancement of science and technology, genome sequencing technology has gradually entered the clinical application of tumors, and many amazing results have em...

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