Method for capturing genetic colorectal cancer genome target sequence

A technology of colorectal cancer and genomics, applied in biochemical equipment and methods, combinatorial chemistry, microbial measurement/testing, etc., can solve the problem of not being able to screen multiple key areas of gene mutation load in a targeted manner and not well satisfying individualization problems such as specific user needs, long single-sample analysis time, etc., to achieve the effect of reducing detection cost and sequencing data volume, reducing non-specific capture, and improving sequencing depth
CN113234822AInactive Publication Date: 2021-08-10XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
Publication Date
2021-08-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of gene detection, and particularly discloses a method for capturing a genetic colorectal cancer genome target sequence, wherein the sequence of a capture probe adopted by the method is as shown in SEQ ID NO: 1-2641, and the capture probe covers a region of 80-300Kb, preferably 80-100Kb, of the genome, and the Tm value of the capture probe is 62-76 DEG C. The probe designed and synthesized by the invention can efficiently and completely capture a coding exon region and an exon-intron junction region of a human genome target region, reduces non-specific capture, is uniform in hybridization temperature, greatly reduces the detection cost and the sequencing data volume, shortens the single sample analysis time, and meets the personalized user requirements; the method is especially suitable for screening of high-risk population of hereditary colorectal cancer and public physical examination markets.
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Description

technical field

[0001] The invention relates to the technical field of gene detection, in particular to a method for capturing genome target sequences of hereditary colorectal cancer. Background technique

[0002] Colorectal cancer is the third most common malignant tumor in the world, and its mortality rate is the second highest, with approximately 1.2 million new patients each year. In China, the morbidity and mortality of colorectal cancer are increasing year by year. About 1 / 3 of patients have a family genetic background, and 5% to 6% of patients can be diagnosed as hereditary colorectal cancer. The awareness of colorectal cancer is insufficient. At present, in addition to several specialized tumor hospitals and a few general hospitals that have carried out related work, there is still a lack of systematic registration and screening of hereditary colorectal cancer. Therefore, the actual diagnosis rate of hereditary colorectal cancer in China is still low. Hereditary co...

Claims

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