Kit and use thereof

Abstract

The invention discloses a kit. The kit comprises a probe, the probe is fixed on a solid matrix or is free in a solution, and the probe can specifically identify at least one part of regions in every gene of seventeen genes in table 1. The invention also provides a use of the kit, a method for constructing a target region sequencing library, a sequencing method, a method for detecting the variation of the target region, and a device for detecting the variation of the target region. The kit and / or the method or the device are / is used to once, simply, conveniently and highly-specifically obtain relevant gene sequences of stomach cancer and accurately detect and analyze the relevant gene sequences in order to make the detection and analysis result used for medication guidance and / or risk predication of the stomach cancer in an auxiliary manner.

Description

technical field [0001] The present invention relates to the field of biomedicine, specifically, to a kit and its use, more specifically, the present invention relates to a kit, the use of the kit, a method for constructing a sequencing library of a target region, a sequencing method, a A method and device for detecting variation in a target region. Background technique [0002] Gastric cancer is one of the most common malignant tumors in the world, ranking second in the global cause of cancer death. In 2012, the number of new patients with gastric cancer in the world was about 950,000, and the number of deaths from gastric cancer was about 700,000. The incidence of gastric cancer is the highest in East Asia. . my country is a country with a high incidence of gastric cancer. According to the data of the National Cancer Center, the incidence and mortality of gastric cancer ranked third among malignant tumors in my country in 2011 [Chen Wanqing, Zheng Rongshou, Zeng Hongmei et...

AI Technical Summary

Problems solved by technology

The PCR-Sanger method has good accuracy and can detect new point mutation types and InDel, but the sensitivity is very low, and it can only detect high-frequency mutations with a mutation rate greater than 20%, and it is difficult to detect multi-gene and multi-site detection

The PCR-Mass method cannot detect unknown mutations, nor can it detect InDel, and the sensitivity is about 5%. This technology has relatively large limitations

The ARMS-PCR method has good specificity and high sensitivity, and can detect 1%-5% low-frequency mutations, but cannot detect unknown mutations, and it is also difficult to simultaneously detect multiple genes and multiple loci

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