Thin-layer chromatography identification method of caulis tinosporae sinensis formula granules
A technology of formula granules and thin-layer chromatography, which is applied in the field of thin-layer identification of wide tendon rattan formula granules, can solve the problems of inapplicability of property identification and lack of medicinal material appearance characteristics, etc., and achieve the goals of simple method, improved quality control, and guaranteed product quality Effect
Inactive Publication Date: 2014-03-26
涂瑶生 +4
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Problems solved by technology
The 2010 edition of the "Chinese Pharmacopoeia" does not include the variety of the broad tendon rattan medicinal material, and the traditional Chinese medicine formula granules no longer have the appearance characteristics of the medicinal material after a series of processes, and the identification of properties is obviously not applicable to the traditional Chinese medicine formula granules
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[0011] Below in conjunction with specific embodiment, the present invention is described in further detail, but does not constitute any restriction to the present invention, anyone makes any immaterial change within the scope of the claims of the present invention, still in the claims of the present invention within the scope of protection.
[0012] Take 0.5g of this product powder, add 30ml of methanol, reflux for 30 minutes, filter, evaporate the filtrate to dryness in a water bath, add 20ml of water to the residue to dissolve, add 20ml of ethyl acetate each time, shake and extract twice, combine the ethyl acetate extract, and put it in a water bath Evaporate ethyl acetate to dryness, add 1ml of methanol to the residue to dissolve it, and use it as the test solution. Take another 5 g of the reference medicinal material of vine vine, add 50 ml of water, boil for 30 minutes, filter, evaporate the filtrate to dryness in a water bath, add 30 ml of methanol to the residue, and pr...
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Abstract
The invention discloses a thin-layer chromatography identification method of caulis tinosporae sinensis formula granules and belongs to the technical field of traditional Chinese medicine quality detection. The method is simple in detection and reliable in detection result, and is technically characterized in that the method sequentially includes the steps: taking 0.5g of caulis tinosporae sinensis formula granule powder, adding solvent for reflux, filtering, collecting filtrate, drying the solvent by distillation, adding water into residue for dissolution, then adding ethyl acetate for extraction, combining ethyl acetate extracting solution, drying the ethyl acetate by distillation, adding 1ml of methanol into residue for dissolution, and using the dissolved residue as test sample solution; separately taking 5g of caulis tinosporae sinensis comparison medicinal materials, adding water, boiling for 30 minutes, filtering, drying filtrate by distillation by means of water bath and adding solvent into residue so that comparison medicinal material solution is prepared by the same method; and testing according to thin-layer chromatography by absorbing 5-microliter of each of the two solutions, developing by the aid of methylbenzene-ethyl acetate-methanoic acid serving as developer, taking out, drying, spraying sulfuric acid and ethanol solution with the concentration of 10%, heating at 105 DEG C until spot color is distinct, and inspecting below an ultraviolet lamp.
Description
technical field [0001] The invention relates to a detection method, in particular to a thin-layer identification method for wide tendon rattan formula granules, which belongs to the technical field of traditional Chinese medicine quality detection. Background technique [0002] Wide tendon rattan formula granule is a formula granule made from the dried cane of the plant Fangjiaceae, Chinese green cow gallbladder. It tastes bitter, slightly cold, enters the liver meridian, has the effects of relaxing tendons, activating collaterals, dispelling wind and relieving pain, and is mainly used for rheumatism Arthralgia, tendon spasm, unfavorable flexion and extension, bruises. Compared with the traditional Chinese medicine decoction, it has the advantages of no need to decoct, easy to take, easy to store, convenient to carry and the like. The 2010 edition of the "Chinese Pharmacopoeia" did not include the variety of the medicinal material of rattan vine, and the traditional Chinese...
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IPC IPC(8): G01N30/90
Inventor 涂瑶生孙冬梅程学仁毕晓黎刘法锦
Owner 涂瑶生