Penicillium paecilomyces cicadae (miquel) samson fermentation product used as feed additive as well as preparation method and application thereof
A feed additive, the technology of Paecilomyces cicadae, applied in animal feed, animal feed, applications, etc., can solve problems such as Paecilomyces cicadae that has not been seen yet, achieve the best production and social benefits, occupy less land, and invest more little effect
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Embodiment 1
[0033] Embodiment 1: the production of Paecilomyces cicadae fermentation product feed additive
[0034] (1) Preparation of inclined-plane strains: boil potatoes, filter to remove slag, add sucrose, constant volume, add agar to melt, put into test tubes, sterilize at 121°C for 0.5h under 0.1Mpa pressure, and deflate completely , placed on an inclined plane while hot to cool naturally. Under sterile conditions, Paecilomyces cicadae was connected to a slant test tube, placed in an incubator at 20°C, and cultivated for 15 days.
[0035] (2) Preparation of the seed solution in shake flasks: boil the potatoes first, filter to remove slag, add sucrose, constant volume, seal and sterilize. Sterilization conditions: under 0.1Mpa pressure, 121 ℃, 0.5h sterilization. After the sterilization is completed, the medium is cooled, and the slant is inoculated into the shake flask medium under aseptic conditions, and the inoculated shake flask is placed in a shaker for shaking culture. Cultu...
Embodiment 2
[0047] Embodiment 2: the production of Paecilomyces cicadae fermentation product feed additive
[0048] (1) Preparation of slant strains: same as in Example 1.
[0049] (2) Preparation of seed liquid: with embodiment 1.
[0050] (3) Solid culture
[0051] a. Preparation of solid phase medium:
[0052] According to the 2# formulation in Table 1, the solid medium was prepared by the same method as in Example 1.
[0053] b. Subpackage of solid-phase medium, sterilization, production of cultivars, germ management, harvesting, dehydration, drying and pulverization are all the same as in Example 1.
Embodiment 3
[0054] Embodiment 3: the production of Paecilomyces cicadae fermentation product feed additive
[0055] (1) Preparation of slant strains: same as in Example 1.
[0056] (2) Preparation of seed liquid: with embodiment 1.
[0057] (3) Solid culture
[0058] a. Preparation of solid phase medium:
[0059] The solid medium was prepared according to the 3# formula in Table 1 using the same method as in Example 1.
[0060] b. Subpackage of solid-phase medium, sterilization, production of cultivars, germ management, harvesting, dehydration, drying and pulverization are all the same as in Example 1.
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