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A Modeling Method for Jointly Evaluating the Bioavailability and Toxicity of Food Cadmium Accumulation in Human

A technology of bioavailability and model establishment, which is applied in the field of model establishment for joint evaluation of food cadmium accumulation on human bioavailability and toxicity, can solve the problems of toxicity evaluation deviation, complicated operation, inaccuracy, etc., and achieve easy test conditions. Control, simple operation, little pollution effect

Inactive Publication Date: 2014-10-15
HENAN UNIV OF SCI & TECH
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the biological effects on the human body are evaluated indirectly mainly by measuring the accumulation of cadmium in food, which inevitably has great inaccuracies; animal experiments are also a method to evaluate food safety, but due to the existence of animal experiments The cycle is long, the operation is complicated, and it is easily affected by experimental factors (such as other feed ingredients, experimental conditions, animal physiological conditions, etc.), so there are often deviations in toxicity evaluation

Method used

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  • A Modeling Method for Jointly Evaluating the Bioavailability and Toxicity of Food Cadmium Accumulation in Human
  • A Modeling Method for Jointly Evaluating the Bioavailability and Toxicity of Food Cadmium Accumulation in Human

Examples

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Embodiment 1

[0024] Example 1: Bioavailability and toxicity of rice cadmium accumulation to human body:

[0025] (1) Collect rice grains, grind them, pass through pepsin digestion solution with pH 2.0, digest in 37°C constant temperature water bath for 1 hour; trypsin-bile extract digestion liquid with pH 7.0, digest in 37°C constant temperature water bath 2h; centrifuge, collect the supernatant, and autoclave the supernatant;

[0026] (2) Evenly mix the supernatant with the culture solution (without cadmium) to make a mixed solution, draw 2.5mL of the mixed solution and add it to the Caco-2 cell layer of the established joint culture model, and at the same time, draw the isotonic culture medium Add 2.5mL of solution to the 293T cell layer, and continue to culture in a carbon dioxide incubator at 37°C for 24 hours; if the food cadmium accumulation is low, a chronic toxicity test can be carried out, and the longest culture can be continued for 10 days;

[0027] (3) Collect Caco-2 cells and...

Embodiment 2

[0030] Example 2: Bioavailability and toxicity of animal liver cadmium accumulation to human body:

[0031] (1) Cook the animal liver, grind it, pass through the pepsin digestion solution with pH 2.0, and digest it in a constant temperature water bath at 37°C for 1 hour; trypsin-bile extract digestion liquid at pH 7.0, and digest it in a constant temperature water bath at 37°C Digest for 2 hours; centrifuge, collect the supernatant, and autoclave the supernatant;

[0032] (2) Evenly mix the supernatant with the culture solution (without cadmium) to make a mixed solution, draw 2.5mL of the mixed solution and add it to the Caco-2 cell layer of the established joint culture model, and at the same time, draw the isotonic culture medium Add 2.5mL of solution to the 293T cell layer, and continue to culture in a carbon dioxide incubator at 37°C for 24 hours; if the food cadmium accumulation is low, a chronic toxicity test can be carried out, and the longest culture can be continued f...

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Abstract

The invention relates to a method for establishing a model for jointly evaluating the bioavailability and toxicity of food cadmium accumulation on the human body, comprising the following steps: (1) digestion of food samples and acquisition of measurement liquid: a. After the food samples are collected, grind them and use gastric juice and intestinal juice in sequence; b. Centrifuge the digestion mixture, collect the supernatant, and sterilize under high pressure; (2) Cultivate Caco-2 cells and 293T cells alone; (3) Combined culture of Caco-2 cells and 293T cells: The insertion tank with Caco-2 cells cultured was moved into a six-well plate with 293T cells cultured, and continued to culture for 24 hours; (4) Evaluation of cadmium bioavailability and toxicity: the food sample assay solution was placed in the Caco-2 cells of the joint culture model At the same time, add isotonic incubation solution on the 293T cell layer and continue to culture for 24 hours; in chronic toxicity test, the longest joint culture can be 10 days; (5) Index detection. The invention has the characteristics of simple operation, easy control of test conditions, less pollution, economy, accurate results, etc., and has the advantages of joint evaluation of biological effectiveness and toxicity. It is suitable for the safety evaluation of cadmium accumulation in grains, vegetables and animal foods.

Description

technical field [0001] The invention relates to a method for establishing a model for jointly evaluating the bioavailability and toxicity of food cadmium accumulation on human body. Background technique [0002] Cadmium pollution has been widespread in the world, which leads to a certain amount of cadmium enrichment in human's main food, which poses a potential threat to human health. Food safety testing is an important guarantee to prevent human cadmium poisoning. At present, the biological effects on the human body are indirectly evaluated mainly by measuring the accumulation of cadmium in food, which inevitably has great inaccuracies; animal experiments are also a method to evaluate food safety, but due to the existence of animal experiments The cycle is long, the operation is complicated, and it is easily affected by experimental factors (such as other feed ingredients, experimental conditions, animal physiological conditions, etc.), so there are often deviations in tox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/02G01N27/62G01N27/626
Inventor 何万领李晓丽张才李健张纪亮李旺李元晓杨盼盼
Owner HENAN UNIV OF SCI & TECH
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