Lupus anticoagulant (LA) screening and determining reagent kit (freezing method)

Abstract

The invention discloses a reagent for screening and determining lupus anticoagulant (LA) in blood plasma by a dilution viper venom test method. The LA screening and determining reagent comprises viper venom blood clotting factor X activating agents, rapeseed phosphatide and calcium ions. The rapeseed phosphatide replaces the animal cephalin in the prior art, the proper addition quantity is low, and the rapeseed phosphatide has higher specificity and sensitivity for LA. The LA screening and determining reagent has the advantages of good specificity, high sensitivity, high stability and high detection feasibility degree.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a reagent for screening and measuring lupus anticoagulant in blood plasma by a dilute viper venom test method. Background technique [0002] Lupus anticoagulant (Lupus anticoagulant, LA) is a phospholipid-dependent pathological circulating anticoagulant substance, which has anticoagulant effect in vitro, and can interact with phospholipids in vivo to produce a procoagulant effect and promote thrombus formation. It has been found to be present in a variety of diseases and its persistence is thought to be associated with unexplained recurrent miscarriages, stillbirths, fetal growth retardation, arteriovenous thrombosis, various thrombophilic disorders, and certain autoimmune disorders danger signal. Therefore, the screening detection of LA is of great significance to the diagnosis of various clinical diseases. [0003] In recent years, a variety of LA screening methods have b...

AI Technical Summary

Problems solved by technology

Most of the phospholipids used in the prior art are animal cephalins, such as bovine cephalin and rabbit cephalin, which tend to reduce the detection rate of lupus anticoagulant because of their low sensitivity

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